文献类型：期刊文献

英文题名：The PagKNAT2/6b-PagBOP1/2a Regulatory Module Controls Leaf Morphogenesis in Populus

作者：Zhao, Yanqiu[1] Zhang, Yifan[1] Zhang, Weilin[1] Shi, Yangxin[1] Jiang, Cheng[1] Song, Xueqin[2] Tuskan, Gerald A.[3] Zeng, Wei[1] Zhang, Jin[1] Lu, Mengzhu[1,2]

第一作者：Zhao, Yanqiu

通信作者：Zhang, J[1];Lu, MZ[1];Lu, MZ[2]

机构：[1]Zhejiang A&F Univ, State Key Lab Subtrop Silviculture, Hangzhou 311300, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat Natl Forestry &, Res Inst Forestry, Beijing 100091, Peoples R China;[3]Oak Ridge Natl Lab, Biosci Div, Ctr Bioenergy Innovat, Oak Ridge, TN 37830 USA

年份：2022

卷号：23

期号：10

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000801319900001)】；

基金：This work was supported by the National Science Foundation of China (32101482), the Zhejiang A&F University Research and Development Fund Talent Startup Project (2018FR013, 2021LFR013).

语种：英文

外文关键词：Populus; leaf morphogenesis; PagKNAT2; 6b; PagBOP1; 2a

摘要：Leaf morphogenesis requires precise regulation of gene expression to achieve organ separation and flat-leaf form. The poplar KNOTTED-like homeobox gene PagKNAT2/6b could change plant architecture, especially leaf shape, in response to drought stress. However, its regulatory mechanism in leaf development remains unclear. In this work, gene expression analyses of PagKNAT2/6b suggested that PagKNAT2/6b was highly expressed during leaf development. Moreover, the leaf shape changes along the adaxial-abaxial, medial-lateral, and proximal-distal axes caused by the mis-expression of PagKNAT2/6b demonstrated that its overexpression (PagKNAT2/6b OE) and SRDX dominant repression (PagKNAT2/6b SRDX) poplars had an impact on the leaf axial development. The crinkle leaf of PagKNAT2/6b OE was consistent with the differential expression gene PagBOP1/2a (BLADE-ON-PETIOLE), which was the critical gene for regulating leaf development. Further study showed that PagBOP1/2a was directly activated by PagKNAT2/6b through a novel cis-acting element "CTCTT". Together, the PagKNAT2/6b-PagBOP1/2a module regulates poplar leaf morphology by affecting axial development, which provides insights aimed at leaf shape modification for further improving the drought tolerance of woody plants.