文献类型：期刊文献

英文题名：Characterization of Arabidopsis thaliana Root-Related Mutants Reveals ABA Regulation of Plant Development and Drought Resistance

作者：Dong, Huan[1,2] Ma, Xiaonan[1] Zhang, Pei[3] Wang, Huan[4] Li, Xiaoli[1] Liu, Jiaxing[1] Bai, Ling[1] Song, Chun-peng[1]

第一作者：Dong, Huan

通信作者：Bai, L[1]

机构：[1]Henan Univ, Inst Plant Stress Biol, Inst Biomed Informat, State Key Lab Cotton Biol,Dept Biol, 85 Minglun St, Kaifeng 475001, Henan, Peoples R China;[2]Henan Agr Univ, Coll Life Sci, Zhengzhou 475000, Henan, Peoples R China;[3]Chinese Acad Forestry, Expt Ctr Trop Forestry, Pingxiang 532600, Guangxi, Peoples R China;[4]Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China

年份：2020

卷号：39

期号：3

起止页码：1393-1401

外文期刊名：JOURNAL OF PLANT GROWTH REGULATION

收录：;Scopus(收录号：2-s2.0-85078808859);WOS:【SCI-EXPANDED(收录号:WOS:000510288100001)】；

基金：This work was supported by the Ministry of Agriculture of China (2016ZX08009-003), the National Natural Science Foundation of China (31430061) and China Postdoctoral Science Foundation (2019M652521). We are grateful to Dr. Jan-Ru Zuo (Institute of Genetics and Developmental Biology, CAS, China) for providing T-DNA insertion mutant library seeds.

语种：英文

外文关键词：Root growth; ABA responses; Seed germination; Drought resistance

摘要：The plant hormone abscisic acid (ABA) regulates many processes of plant growth and development. ABA receptors have been identified in studies of the ABA response of stomatal movement, but the underlying mechanisms of ABA-regulated root growth and development are unclear. To address these questions, we screened for Arabidopsis thaliana mutants based on the sensitivity of root growth to exogenous ABA, using ethyl methanesulfonate-mutagenized (EMS) and T-DNA insertion mutant libraries. We identified 11 mutants, termed roa1-roa11, with Root growth Overly sensitive to ABA (ROA) phenotypes, and cloned two of the ROA genes, one by thermal asymmetric interlaced PCR technology (ROA3) and the other by map-based cloning (ROA9). The roa mutants were also found to have defects in other major ABA responses, including ABA-mediated seed germination and drought resistance. The roa mutants provide crucial genetic material for further studies of ABA signaling and regulatory mechanisms in root growth and development.