文献类型：期刊文献

英文题名：The conserved salt-responsive genes in the roots of Populus x canescens and Arabidopsis thaliana

作者：Luo, Jie[1,2] Shi, Wenguang[1] Li, Hong[3] Janz, Dennis[4] Luo, Zhi-Bin[1,4]

第一作者：Luo, Jie

通信作者：Luo, ZB[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Key Lab Silviculture State Forestry Adm, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China;[3]Chinese Acad Forestry, Postgrad Sch, Beijing 100091, Peoples R China;[4]Univ Gottingen, Dept Forest Bot & Tree Physiol, Busgen Inst, Busgenweg 2, D-37077 Gottingen, Germany

年份：2016

卷号：129

起止页码：48-56

外文期刊名：ENVIRONMENTAL AND EXPERIMENTAL BOTANY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000379797400006)】；

基金：This work was jointly supported by the Special Fund for Forest Scientific Research in the Public Welfare (Grant no. 201504101), the National Natural Science Foundation of China (Grant nos. 31270647 and 31470618), Deutsche Forschungsgemeinschaft (grants to A.P. and H.R. via FOR496 [Poplar Research Group, Germany]), and the Deutscher Akademischer Austauschdienst (postdoctoral scholarship to Z.-B.L. in the program Modern Application of Biotechnology). We thank Prof. Andrea Polle for her valuable suggestions during the preparation of this manuscript.

语种：英文

外文关键词：Transcriptome; Salinity; Populus; Co-expression; Hub genes; cis-Elements

摘要：To dissect the salt responsive genes in the roots of Populus x canescens after salt exposure and to characterize the conserved transcripts with differential expression in the roots of P. x canescens and Arabidopsis thaliana under salinity, P. x canescens grown in sandy soil was exposed to 150 mM NaCl for 18 days and the transcriptional profiling was analyzed in the roots by using a whole genome poplar array chip. The raw data of the Affymetrix Arabidopsis genome arrays were obtained from the online array data bank and statistically analysed as that of the poplar. In the roots of P. x canescens exposed to salinity, about 860 genes displayed significantly differential expression, including 647 up-regulated and 213 down-expressed genes. In the roots of A. thaliana,1292 genes were up- and 718 down-expressed. Among the differentially expressed genes between P. x canescens and A. thaliana, a set of common genes (128 genes) showed the same change pattern in response to salinity, including 114 induced and 14 repressed genes. A salt-responsive co-expression network was constructed with 98 common genes. Among the co-expressed genes, 22 genes were defined as hub genes which were involved in fundamental biological processes such as abiotic stimulus and signal transduction. Moreover, the cis-regulatory elements were found in the conserved motifs of hub genes. These results suggest that P. x canescens and A. thaliana possess conserved salt-responsive genes and that cis-elements in the conserved motifs of hub genes play a crucial role in coordinating the co-expression of the common genes underlying the physiological acclimation to salinity in the roots of P. x canescens and A. thaliana. (C) 2015 Elsevier B.V. All rights reserved.