文献类型：期刊文献

英文题名：Association mapping revealed phenolic content-related SNPs and haplotypes in pecan (Carya illinoinensis)

作者：Zhang, Chengcai[1] Shao, Weizhong[2] Ren, Huadong[1] Shen, Fengqiang[3] Xu, Qiangwei[3] Chang, Jun[] Wang, Kailiang[1] Yao, Xiaohua[1]

第一作者：张成才

通信作者：Yao, XH[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding Zhejiang Prov, Hangzhou 311400, Peoples R China;[2]Forestry Bur Jiande, Jiande 311600, Zhejiang, Peoples R China;[3]Zhejiang Mat & Prod Changle Ind Co Ltd, Hangzhou 311100, Peoples R China

年份：2024

卷号：220

期号：12

外文期刊名：EUPHYTICA

收录：;Scopus(收录号：2-s2.0-85211188314);WOS:【SCI-EXPANDED(收录号:WOS:001366261200001)】；

基金：This work was supported by the National Natural Science Foundation of China No. 31800575 and the Key Scientific and Technological Grant of Zhejiang for Breeding New Agricultural Varieties No. 2021C02066-12.

语种：英文

外文关键词：Pecan; Phenolics; Molecular marker-assisted selection; SNP; Association analysis

摘要：Phenolics are essential quality components in pecan nuts, influencing the nutritional value of various varieties. Conventional breeding methods for pecan trees are time-consuming and laborious due to their considerable height and extended juvenile period. Molecular marker-assisted selection offers the potential to expedite the breeding process. In this study, we examined the allelic variations of five phenolics biosynthesis-related genes within a germplasm population. The linkage disequilibrium (LD) values (r(2)) between single nucleotide polymorphisms (SNPs) of each gene were analyzed, demonstrating a decrease in r(2) to less than 0.2 within a 1.0 kb distance. The contents of total phenolics, total flavonoids, and condensed tannins in the kernels of each individual were assessed over two consecutive years. Following this, association mapping was conducted. A total of 29 significant marker-trait pairs and eight significant haplotype-trait pairs were observed. Among these, three SNPs in CiPAL4 and two haplotypes in CiCHS3 were consistently identified over both years. The explanation rates of the SNPs for the observed traits ranged from 3.45 to 14.39% (p < 0.05). This study offers valuable insights into the molecular basis underlying phenolic biosynthesis in pecan trees, which will enhance the efficiency of quality improvement in this plant and other species within the Carya genus.