文献类型：期刊文献

英文题名：OsPAP26 Encodes a Major Purple Acid Phosphatase and Regulates Phosphate Remobilization in Rice

作者：Gao, Wenwen[1] Lu, Linghong[1] Qiu, Wenmin[1,2] Wang, Chuang[1,3] Shou, Huixia[1]

第一作者：Gao, Wenwen

通信作者：Shou, HX[1]

机构：[1]Zhejiang Univ, Coll Life Sci, State Key Lab Plant Physiol & Biochem, Hangzhou 310058, Zhejiang, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Breeding Zhejiang Prov, Hangzhou 311400, Zhejiang, Peoples R China;[3]Huazhong Agr Univ, Coll Resources & Environm, Wuhan 430070, Peoples R China

年份：2017

卷号：58

期号：5

起止页码：885-892

外文期刊名：PLANT AND CELL PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000402853400003)】；

基金：This work was supported by the Ministry of Science and Technology of China [2016YFD0100703, 2016ZX08003005-001 and 2014ZX08009328-002]; the National Natural Science Foundation of China [31572189, 31401934 and 31471929]; the Ministry of Education of China [111 project; B14027]; and the Natural Science Foundation of Zhejiang Province [LZ16C150001].

语种：英文

外文关键词：Acid phosphatase; Organic phosphorus; OsPAP26; Phosphate remobilization; Rice

摘要：During phosphate (Pi) starvation or leaf senescence, the accumulation of intracellular and extracellular purple acid phosphatases (PAPs) increases in plants in order to scavenge organic phosphorus (P). In this study, we demonstrated that a PAP-encoding gene in rice, OsPAP26, is constitutively expressed in all tissues. While the abundance of OsPAP26 transcript is not affected by Pi supply, it is up-regulated during leaf senescence. Furthermore, Pi deprivation and leaf senescence greatly increased the abundance of OsPAP26 protein. Overexpression or RNA interference (RNAi) of OsPAP26 in transgenic rice significantly increased or reduced APase activities, respectively, in leaves, roots and growth medium. Compared with wild-type (WT) plants, Pi concentrations of OsPAP26-overexpressing plants increased in the non-senescing leaves and decreased in the senescing leaves. The increased remobilization of Pi from the senescing leaves to non-senescing leaves in the OsPAP26-overexpressing plants resulted in better growth performance when plants were grown in Pi-depleted condition. In contrast, OsPAP26RNAi plants retained more Pi in the senescing leaves, and were more sensitive to Pi starvation stress. OsPAP26 was found to localize to the apoplast of rice cells. Western blot analysis of protein extracts from callus growth medium confirmed that OsPAP26 is a secreted PAP. OsPAP26-overexpressing plants were capable of converting more ATP into inorganic Pi in the growth medium, which further supported the potential role of OsPAP26 in utilizing organic P in the rhizosphere. In summary, we concluded that OsPAP26 performs dual functions in plants: Pi remobilization from senescing to non-senescing leaves; and organic P utilization.