文献类型：期刊文献

英文题名：High-Quality Genome Assembly of Olea europaea subsp. cuspidata Provides Insights Into Its Resistance to Fungal Diseases in the Summer Rain Belt in East Asia

作者：Wang, Li[1] Zhang, Jianguo[1,2] Peng, Dan[3] Tian, Yang[1] Zhao, Dandan[1] Ni, Wanning[1] Long, Jinhua[1] Li, Jinhua[1] Zeng, Yanfei[1,2] Wu, Zhiqiang[3] Tang, Yiyun[4] Wang, Zhaoshan[1,2]

通信作者：Zhang, JG[1];Wang, ZS[1];Zhang, JG[2];Wang, ZS[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Key Lab Silviculture State Forestry Adm, Beijing, Peoples R China;[2]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry Southe, Nanjing, Peoples R China;[3]Kunpeng Inst Modern Agr Foshan, Foshan, Peoples R China;[4]Nujiang State Forestry & Grassland Bur Yunnan Prov, Ecol Restorat & Ind Dev Workstat, Kunming, Peoples R China

年份：2022

卷号：13

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;Scopus(收录号：2-s2.0-85131530316);WOS:【SCI-EXPANDED(收录号:WOS:000805105800001)】；

基金：Financial support for this research was provided by the National Key R & D Program of China (No. 2019YFD1000602).

语种：英文

外文关键词：genome assembly; Olea europaea; susceptibility gene; demographic history; nature selection

摘要：The olive tree (Olea europaea L.) is the most iconic fruit crop of the Mediterranean Basin. Since the plant was introduced to China in the 1960s, the summer rain climate makes it susceptible to pathogens, leading to some olive diseases. Olea europaea L. subsp. cuspidata is natively distributed in the Yunnan province of China. It has a smaller fruit size, lower oil content, and higher resistance compared to subsp. europaea, which makes subsp. cuspidata a critical germplasm resource to be investigated. Here, a high-quality genome of subsp. cuspidata with 1.38 Gb in size was assembled and anchored onto 23 pseudochromosomes with a mounting rate of 85.57%. It represents 96.6% completeness [benchmarking universal single-copy orthologs (BUSCO)] with a contig N50 of 14.72 Mb and a scaffold N50 of 52.68 Mb, which shows a significant improvement compared with other olive genomes assembled. The evaluation of the genome assembly showed that 92.31% of resequencing reads and an average of 96.52% of assembled transcripts could be aligned to the assembled genome. We found that a positively selected gene, evm.model.Chr16.1133, was shared with the results of transcriptome analysis. This gene belongs to the susceptible gene and negatively regulates the disease resistance process. Furthermore, we identified the Cercospora genus which causes the leaf spot disease in the infected leaves. The high-quality chromosome-level genomic information presented here may facilitate the conservation and utilization of germplasm resources of this subspecies and provide an essential genetic basis for further research into the differences in oil content and resistance between subsp. cuspidata and europaea.