文献类型：期刊文献

中文题名：尾巨桉EuNF-YB1基因克隆、亚细胞定位及表达特异性分析

英文题名：Cloning,subcellular localization and expression pattern analysis of EuNF-YB1 gene in Eucalyptus urophylla×Eucalyptus grandis

作者：李娟[1,2] 庄莉[1] 徐艳利[1] 李光友[2] 徐建民[2] 陆钊华[2]

第一作者：李娟

机构：[1]山西师范大学生命科学学院,山西太原030031;[2]中国林业科学研究院热带林业研究所,广东广州510520

年份：2025

卷号：45

期号：5

起止页码：123-131

中文期刊名：中南林业科技大学学报

外文期刊名：Journal of Central South University of Forestry & Technology

收录：;北大核心:【北大核心2023】；

基金：国家自然科学青年基金项目(32201524);广州市基础与应用基础研究专题项目(2023A04J0711)。

语种：中文

中文关键词：尾巨桉;EuNF-YB1;生物信息学;亚细胞定位;表达分析

外文关键词：Eucalyptus urophylla×Eucalyptus grandis;EuNF-YB1;bioinformatics;subcellular localization;expression analysis

分类号：S718.43

摘要：【目的】尾巨桉Eucalyptus urophylla×E.grandis作为重要的用材树种,具有广泛的用途和经济价值。通过克隆、生物信息学分析、亚细胞定位和组织特异表达分析方法对核转录因子EuNF-YB1进行研究,为深入了解其基本特征及在尾巨桉生长发育中的调控作用提供理论基础。【方法】以尾巨桉优良无性系DH3229的6月龄幼苗为试验材料,通过PCR技术,克隆获得EuNF-YB1,并通过生物信息学、亚细胞定位和组织特异性表达对其进行综合分析。【结果】EuNF-YB1基因编码区长度为534 bp,编码178个氨基酸,其分子量为19.10 kD,等电点为6.44,属于不稳定的亲水性蛋白。多序列比对分析表明,EuNF-YB1含有保守的DNA结合结构域、NF-YA的互作结构域及NF-YC的互作结构域,是一个典型的NF-YB转录因子。进化关系分析表明,EuNF-YB1与拟南芥AtNF-YB1和菊花CmNF-YB8蛋白有较近的亲缘关系。亚细胞定位试验结果表明,该蛋白定位于细胞核和细胞质中。组织特异性表达结果显示,EuNF-YB1基因在各组织中均有表达,尤其在花中表达水平最高。因此,推测EuNF-YB1基因在桉树生长发育尤其是开花调控中可能发挥着重要作用。【结论】尾巨桉EuNF-YB1基因的克隆及表达分析,揭示了EuNF-YB1基因在桉树生长发育尤其是开花调控中可能发挥着重要作用。
【Objective】Eucalyptus urophylla×E.grandis,recognized as a significant timber species,possesses extensive applications and considerable economic value.In this study,cloning,bioinformatics analysis,subcellular localization and tissue-specific expression profiling were employed to investigate the nuclear transcription factor EuNF-YB1.This research provides a theoretical basis for understanding its basic characteristics and its regulatory role in the growth and development of Eucalyptus urophylla×E.grandis.【Method】EuNF-YB1 was obtained from DH3229 clones of Eucalyptus urophylla×E.grandis at 6 months of age by PCR.The clones were analyzed by bioinformatics,subcellular localization and tissue-specific expression.【Result】EuNF-YB1 gene coding region was 534 bp,encoding 178 amino acids with a molecular weight of 19.10 kD and pI 6.44,which belongs to the unstable hydrophilic proteins.Multiple sequence alignment analysis showed that EuNF-YB1 also contained conserved DNA-binding domains,NF-YA interaction domains and NF-YC interaction domains,indicating that EuNF-YB1 was also a typical NF-YB transcription factor.Evolutionary relationship analysis showed that EuNF-YB1 was closely related to AtNF-YB1 and CmNF-YB8 protein.The results of subcellular localization showed that the protein was localized in the nucleus and cytoplasm.Tissue-specific expression analysis showed that EuNF-YB1 gene was expressed in all tissues,with the highest expression in flowers.【Conclusion】The cloning and expression analysis of the EuNF-YB1 gene in Eucalyptus urophylla×E.grandis indicated that this gene may play a significant role in the growth and development of Eucalyptus,particularly in the regulation of flowering.