文献类型：期刊文献

英文题名：Desiccation Treatment and Endogenous IAA Levels Are Key Factors Influencing High Frequency Somatic Embryogenesis in Cunninghamia lanceolata (Lamb.) Hook

作者：Zhou, Xiaohong[1,2] Zheng, Renhua[3] Liu, Guangxin[1,2] Xu, Yang[1,6] Zhou, Yanwei[1,2] Laux, Thomas[4] Zhen, Yan[1,2] Harding, Scott A.[5] Shi, Jisen[1,2] Chen, Jinhui[1,2]

第一作者：Zhou, Xiaohong

通信作者：Shi, JS[1];Chen, JH[1];Shi, JS[2];Chen, JH[2]

机构：[1]Nanjing Forestry Univ, Minist Educ, Key Lab Forest Genet & Biotechnol, Nanjing, Jiangsu, Peoples R China;[2]Nanjing Forestry Univ, Coll Forestry, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing, Jiangsu, Peoples R China;[3]Fujian Acad Forestry, State Forestry Adm, Engn Res Ctr Chinese Fir, Key Lab Timber Forest Breeding & Cultivat Mountai, Fuzhou, Fujian, Peoples R China;[4]Albert Ludwigs Univ Freiburg, Fac Biol, Ctr Biol Signaling Studies, Freiburg, Germany;[5]Univ Georgia, Sch Forestry & Nat Resources, Athens, GA 30602 USA;[6]Chinese Acad Forestry, Res Inst Subtrop Forestry, Fuyang, Peoples R China

年份：2017

卷号：8

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000417036700001)】；

基金：This work was supported by National High Technology Research and Development Program of China (863 Program, 2013AA102705), Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP, 20113204130002), Natural Science Foundation of Jiangsu University grant 13KJA220001, Talent Project by the Ministry of Science and Technology, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, and Priority Academic Program Development of Jiangsu Higher Education Institutions.

语种：英文

外文关键词：ABA; desiccation; endogenous hormone; PEG; somatic embryo; SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK); WUSCHEL homeobox (WOX)

摘要：Cunninghamia lanceolata (Lamb.) Hook (Chinese fir) is an important tree, commercially and ecologically, in southern China. The traditional regenerating methods are based on organogenesis and cutting propagation. Here, we report the development of a high-frequency somatic embryogenesis (SE) regeneration system synchronized via a liquid culture from immature zygotic embryos. Following synchronization, PEM II cell aggregates were developmentally equivalent in appearance to cleaved zygotic embryos. Embryo and suspensor growth and subsequent occurrence of the apical and then the cotyledonary meristems were similar for zygotic and SE embryo development. However, SE proembryos exhibited a more reddish coloration than zygotic proembryos, and SE embryos were smaller than zygotic embryos. Mature somatic embryos gave rise to plantlets on hormone-free medium. For juvenile explants, low concentrations of endogenous indole-3-acetic acid in initial explants correlated with improved proembryogenic mass formation, and high SE competency. Analysis of karyotypes and microsatellites detected no major genetic variation in the plants regenerated via SE, and suggest a potential in the further development of this system as a reliable methodology for true-to-type seedling production. Treatment with polyethylene glycol (PEG) and abscisic acid (ABA) were of great importance to proembryo formation and complemented each other. ABA assisted the growth of embryonal masses, whereas PEG facilitated the organization of the proembryo-like structures. SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) and the WUSCHEL homeobox (WOX) transcription factor served as molecular markers during early embryogenesis. Our results show that ClSERKs are conserved and redundantly expressed during SE. SERK and WOX transcript levels were highest during development of the proembryos and lowest in developed embryos. ClWOX13 expression correlates with the critical transition from proembryogenic masses to proembryos. Both SERK and WOX expression reveal their applicability in Chinese fir as markers of early embryogenesis. Overall, the findings provided evidence for the potential of this system in high fidelity Chinese fir seedlings production. Also, SE modification strategies were demonstrated and could be applied in other conifer species on the basis of our hormonal, morphological and molecular analyses.