文献类型：期刊文献

英文题名：Available nitrogen is the key factor influencing soil microbial functional gene diversity in tropical rainforest

作者：Cong, Jing[1,2,3] Liu, Xueduan[1] Lu, Hui[2,3] Xu, Han[4] Li, Yide[4] Deng, Ye[5] Li, Diqiang[2,3] Zhang, Yuguang[2,3]

第一作者：Cong, Jing

通信作者：Zhang, YG[1]

机构：[1]Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China;[2]Chinese Acad Forestry, Inst Forestry Ecol Environm & Protect, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, State Forestry Adm, Key Lab Forest Ecol & Environm, Beijing 100091, Peoples R China;[4]Chinese Acad Forestry, Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China;[5]Chinese Acad Sci, Ecoenvironm Sci Res Ctr, Beijing 100085, Peoples R China

年份：2015

卷号：15

期号：1

外文期刊名：BMC MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-84939525471);WOS:【SCI-EXPANDED(收录号:WOS:000359689900001)】；

基金：This research was supported by the public welfare project of the national scientific research institution (Grant No. CAFYBB2011004, CAFRIFEEP201101), China, and the National Biological Specimens and Resources Sharing Platform in Nature Reserve (Grant No. 2005DKA21404), and the Strategic Priority Research Program of the Chinese Academy of Sciences (Grant No. XDB15010302), and also supported by Graduate student research innovation project in Hunan province (CX2014B095). We also gratefully acknowledge Dr. Guangliang Li for helping survey the plant plots.

语种：英文

外文关键词：Tropical rainforest; GeoChip; Microbial functional gene diversity; Microbial metabolic potential; Available nitrogen

摘要：Background: Tropical rainforests cover over 50 % of all known plant and animal species and provide a variety of key resources and ecosystem services to humans, largely mediated by metabolic activities of soil microbial communities. A deep analysis of soil microbial communities and their roles in ecological processes would improve our understanding on biogeochemical elemental cycles. However, soil microbial functional gene diversity in tropical rainforests and causative factors remain unclear. GeoChip, contained almost all of the key functional genes related to biogeochemical cycles, could be used as a specific and sensitive tool for studying microbial gene diversity and metabolic potential. In this study, soil microbial functional gene diversity in tropical rainforest was analyzed by using GeoChip technology. Results: Gene categories detected in the tropical rainforest soils were related to different biogeochemical processes, such as carbon (C), nitrogen (N) and phosphorus (P) cycling. The relative abundance of genes related to C and P cycling detected mostly derived from the cultured bacteria. C degradation gene categories for substrates ranging from labile C to recalcitrant C were all detected, and gene abundances involved in many recalcitrant C degradation gene categories were significantly (P < 0.05) different among three sampling sites. The relative abundance of genes related to N cycling detected was significantly (P < 0.05) different, mostly derived from the uncultured bacteria. The gene categories related to ammonification had a high relative abundance. Both canonical correspondence analysis and multivariate regression tree analysis showed that soil available N was the most correlated with soil microbial functional gene structure. Conclusions: Overall high microbial functional gene diversity and different soil microbial metabolic potential for different biogeochemical processes were considered to exist in tropical rainforest. Soil available N could be the key factor in shaping the soil microbial functional gene structure and metabolic potential.