文献类型：期刊文献

英文题名：Concerted control of the L alpha RAV1-L alpha CDKB1;3 module by temperature during dormancy release and reactivation of larch

作者：Li, Wan-Feng[1] Kang, Yanhui[1] Zhang, Yao[2] Zang, Qiao-Lu[1] Qi, Li-Wang[1]

第一作者：李万峰

通信作者：Li, WF[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,Natl Forestry &, 1 Dongxiaofu,Xiangshan Rd, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,Natl Forestry &, 1 Dongxiaofu,Xiangshan Rd, Beijing 100091, Peoples R China

年份：2021

卷号：41

期号：10

起止页码：1918-1937

外文期刊名：TREE PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000710987200012)】；

基金：The National Natural Science Foundation of China (Grant/Award Number: 31770714); the Basic Research Fund of Research Institute of Forestry (Grant/Award Number: RIF2014-07); and the National Transgenic Major Program (Grant/Award Number: 2018ZX08020-003).

语种：英文

外文关键词：cambium; cell-cycle gene; dormancy release; larch; reactivation; temperature; transcription factor

摘要：Dormancy release and reactivation of temperate-zone trees involve the temperature-modulated expression of cell-cycle genes. However, information on the detailed regulatory mechanism is limited. Here, we compared the transcriptomes of the stems of active and dormant larch trees, emphasizing the expression patterns of cell-cycle genes and transcription factors and assessed their relationships and responses to temperatures. Twelve cell-cycle genes and 31 transcription factors were strongly expressed in the active stage. Promoter analysis suggested that these 12 genes might be regulated by transcription factors from 10 families. Altogether, 73 cases of regulation between 16 transcription factors and 12 cell-cycle genes were predicted, while the regulatory interactions between L alpha MYB2O and L alpha CYCB1;1, and L alpha RAV1 and L alpha CDKB1;3 were confirmed by yeast one-hybrid and dual-luciferase assays. Last, we found that LaRAV1 and L alpha CDKB1;3 had almost the same expression patterns during dormancy release and reactivation induced naturally or artificially by temperature, indicating that the LaRAV1-L alpha CDKB1;3 module functions in the temperature-modulated dormancy release and reactivation of larch trees. These results provide new insights into the link between temperature and cell-cycle gene expression, helping to understand the temperature control of tree growth and development in the context of climate change.