文献类型：期刊文献

英文题名：Self-cleaning paper-based microfluidic biosensor employing DNAzyme and semiconducting single-walled carbon nanotube for copper ion detection

作者：Hou, Xiaopeng[1] Cheng, Qiongyi[2] Wang, Hui[1,3]

第一作者：侯晓鹏

通信作者：Wang, H[1]

机构：[1]Chinese Acad Forestry, Res Inst Wood Ind, Beijing 100091, Peoples R China;[2]Acad Agr Planning & Engn, 41 Maizidian St, Beijing 100125, Peoples R China;[3]Chinese Acad Agr Sci, State Key Lab Anim Nutr & Feeding, Inst Anim Sci, Beijing 100193, Peoples R China

年份：2024

卷号：155

外文期刊名：BIOELECTROCHEMISTRY

收录：;EI(收录号：20234715093182);Scopus(收录号：2-s2.0-85177226992);WOS:【SCI-EXPANDED(收录号:WOS:001112418100001)】；

基金：This research was funded by National Natural Science Foundation of China (No.32202738) .

语种：英文

外文关键词：Copper pollution; Chemiresistive; Superhydrophobic paper; Paper-based electrode; Electrochemical biosensor

摘要：Microfluidic paper-based analytical device (mu PAD) offers a simple and efficient platform for point-of-care monitoring, which can be beneficial for copper determination in livestock feed and manure. However, common cellulose paper has excellent hydrophilicity, causing mu PAD is accompanied by poor mechanical properties, short service life, and low sensitivity. Here, a self-cleaning paper-based microfluidic biosensor for Cu2+ determination was proposed to overcome the mentioned shortages in the application. Polymeric octadecyl trichlorosilane was synthesized and decorated on cellulose paper to form hydrophobic paper, which can improve the hydrophobicity, self-cleaning, and pollution ability. In addition, hydrophobic paper, semiconducting singlewalled carbon nanotube, and DNAzyme through the chemical bond were employed to fabricate a self-cleaning paper-based microfluidic biosensor. The properties were investigated using scanning electron microscopy, Raman, and electrochemical methods. The detecting parameters were also optimized. It could measure the Cu2+ concentration from 1 nM to 100 mu M, and the detection limit was 0.65 nM. The self-cleaning paper-based microfluidic biosensor was applied to detect Cu2+ concentration in livestock feed and manure that can meet the requirements for fast screening and detection.