文献类型：期刊文献

英文题名：A full-length transcriptome and gene expression analysis reveal genes and molecular elements expressed during seed development in Gnetum luofuense

作者：Deng, Nan[1,2] Hou, Chen[3,4] He, Boxiang[3,4] Ma, Fengfeng[1,2] Song, Qingan[1,2] Shi, Shengqing[5] Liu, Caixia[1] Tian, Yuxin[1,2]

第一作者：Deng, Nan

通信作者：Liu, CX[1];Tian, YX[1];Tian, YX[2]

机构：[1]Hunan Acad Forestry, 658 Shaoshan Rd, Changsha 410004, Peoples R China;[2]Hunan Cili Forest Ecosyst State Res Stn, Changsha 410004, Hunan, Peoples R China;[3]Guangdong Acad Forestry, Guangzhou 510520, Peoples R China;[4]Guangdong Acad Forestry, Guangdong Prov Key Lab Silviculture Protect & Uti, Guangzhou 510520, Peoples R China;[5]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, 1 Dongxiaofu,Xiangshan Rd, Beijing 100091, Peoples R China

年份：2020

卷号：20

期号：1

外文期刊名：BMC PLANT BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000595832400003)】；

基金：This work was supported by the Special Fund for Hunan Forestry Science and Technology (XLK201812 to N.D.), Forestry Science and Technology Innovation Project of Hunan Province (HNGYL-2019-01 to N.D.), and the Natural Science Foundation of Guangdong Province, China (No. 2018A0303130163 to C.H.). The funding body had no influence over the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.

语种：英文

外文关键词：Gnetales; Full-length transcriptome; Functional genes; Seed; lncRNA

摘要：BackgroundGnetum is an economically important tropical and subtropical gymnosperm genus with various dietary, industrial and medicinal uses. Many carbohydrates, proteins and fibers accumulate during the ripening of Gnetum seeds. However, the molecular mechanisms related to this process remain unknown.ResultsWe therefore assembled a full-length transcriptome from immature and mature G. luofuense seeds using PacBio sequencing reads. We identified a total of 5726 novel genes, 9061 alternative splicing events, 3551 lncRNAs, 2160 transcription factors, and we found that 8512 genes possessed at least one poly(A) site. In addition, gene expression comparisons of six transcriptomes generated by Illumina sequencing showed that 14,323 genes were differentially expressed from an immature stage to a mature stage with 7891 genes upregulated and 6432 genes downregulated. The expression of 14 differentially expressed transcription factors from the MADS-box, Aux/IAA and bHLH families was validated by qRT-PCR, suggesting that they may have important roles in seed ripening of G. luofuense.ConclusionsThese findings provide a valuable molecular resource for understanding seed development of gymnosperms.