文献类型：期刊文献

英文题名：DNA Barcode Authentication and Library Development for the Wood of Six Commercial Pterocarpus Species: the Critical Role of Xylarium Specimens

作者：Jiao, Lichao[1,2] Yu, Min[1,2] Wiedenhoeft, Alex C.[3,4,5,6] He, Tuo[1,2] Li, Jianing[7] Liu, Bo[1,2] Jiang, Xiaomei[1,2] Yin, Yafang[1,2,4]

第一作者：焦立超

通信作者：Yin, YF[1];Yin, YF[2];Yin, YF[3]

机构：[1]Chinese Acad Forestry, Chinese Res Inst Wood Ind, Dept Wood Anat & Utilizat, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Wood Collect WOODPEDIA, Beijing 100091, Peoples R China;[3]USDA Forest Serv, Ctr Wood Anat Res, Forest Prod Lab, Madison, WI 53726 USA;[4]Univ Wisconsin, Dept Bot, Madison, WI 53706 USA;[5]Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA;[6]Univ Estadual Paulista, Ciencias Biol Bot, Botucatu, SP, Brazil;[7]Chinese Acad Trop Agr Sci, Rubber Res Inst, Haikou 571737, Hainan, Peoples R China

年份：2018

卷号：8

期号：1

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000423663100024)】；

基金：This work was supported financially by Fundamental Research Funds of CAF (Grant No. CAFYBB2017MA013), National Natural Science Foundation of China (Grant No. 31600451), Fundamental Research Funds of CAF (Grant No. CAFYBB2017ZE003), China Postdoctoral Science Foundation (Grant No. 2016M590152), and the China Scholarship Council (Grant No. 2016-3035). We would like to express our gratitude to Mr. Di Xiao and Mr. Changyu Xu for the help on collecting samples, and to Sarah Friedrich, Department of Botany, University of Wisconsin-Madison for her technical assistance with the specimen origin and phylogram figures.

语种：英文

摘要：DNA barcoding has been proposed as a useful tool for forensic wood identification and development of a reliable DNA reference library is an essential first step. Xylaria (wood collections) are potentially enormous data repositories if DNA information could be extracted from wood specimens. In this study, 31 xylarium wood specimens and 8 leaf specimens of six important commercial species of Pterocarpus were selected to investigate the reliability of DNA barcodes for authentication at the species level and to determine the feasibility of building wood DNA barcode reference libraries from xylarium specimens. Four DNA barcodes (ITS2, matK, ndhF-rpl32 and rbcL) and their combination were tested to evaluate their discrimination ability for Pterocarpus species with both TaxonDNA and tree-based analytical methods. The results indicated that the combination barcode of matK + ndhF-rpl32 + ITS2 yielded the best discrimination for the Pterocarpus species studied. The mini-barcode ndhF-rpl32 (167-173 bps) performed well distinguishing P. santalinus from its wood anatomically inseparable species P. tinctorius. Results from this study verified not only the feasibility of building DNA barcode libraries using xylarium wood specimens, but the importance of using wood rather than leaves as the source tissue, when wood is the botanical material to be identified.