文献类型：期刊文献

中文题名：金花茶查尔酮异构酶基因全长克隆与表达的初步研究

英文题名：Cloning and Expression Analysis of Chalcone Isomerase Gene cDNA from Camellia nitidissima

作者：周兴文[1,2] 李纪元[1] 范正琪[1]

第一作者：周兴文

机构：[1]中国林业科学研究院亚热带林业研究所;[2]广西玉林师范学院

年份：2012

卷号：25

期号：1

起止页码：93-99

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

基金：国家林业公益性行业科研专项(200704028);国家林业局"948"引进项目(2007-4-04);国家国际合作项目(2011DFA30490)

语种：中文

中文关键词：金花茶;查尔酮异构酶基因;序列分析;相对定量PCR;表达特性

外文关键词：Camellia nitidissima; chalcone isomerase gene; sequence analysis; relative real-time PCR; expression character

分类号：S718.46

摘要：利用RT-PCR和RACE技术,从我国珍稀濒危植物金花茶花瓣中获得了查尔酮异构酶(Chalcone isomerase,CHI)基因的cDNA全长,命名为Cn-CHI,GenBank登录号HQ269805.1。碱基序列分析表明,Cn-CHI基因全长953bp,包含56 bp的5’非翻译区、204 bp的3’非翻译区和一个长为693 bp编码230个氨基酸的开放阅读框。氨基酸序列比对分析表明,Cn-CHI基因编码蛋白与蔷薇科、杜鹃花科、茄科等植物的CHI蛋白同源性都在75%以上,与山茶科山茶属茶树CHI蛋白同源性高达99%。相对荧光定量PCR分析表明,Cn-CHI基因在金花茶花蕾发育过程中呈现先急剧上升后平缓下降的趋势;在花器官的不同部位中,Cn-CHI基因表达量最高的是雌蕊,其次是雄蕊,在萼片和花瓣中的表达量较低且相差不大。
A full-length cDNA sequence of chalcone isomerase（CHI） gene was obtained from petals of Camellia nitidissima using the methods of Reverse Transcription PCR and RACE,named Cn-CHI（GenBank accession No.HQ269805.1）.Sequence analysis indicated that Cn-CHI is 953bp in full length and contains a 5′-untranslated region（5′-UTR） of 56 bp,a 3′-UTR of 204 bp,and an opening reading frame（ORF） of 693bp encoding a 230 predicted amino acids.Sequence alignment of amino acids revealed that Cn-CHI shared more than 75% homology with CHI from plants of Rosaceae,Ericaceae,Solanaceae and 99% homology with C.sinensis.Relative real-time PCR analysis indicated that Cn-CHI showed the tendency of a sharp increase at the early stage and then decreased slowly;the highest transcript abundance in carpels,and the second in stamens,the transcript of Cn-CHI in sepals and petals were almost at the same level.