文献类型：期刊文献

英文题名：A cation-assisted purification strategy for enzymatic hydrolysis lignin via selective chelation and precipitation

作者：Jiang, Jie[1] Ouyang, Chengsi[1] Meng, Haiyang[1] Sun, Hao[2] Chen, Changzhou[1] Jiang, Jianchun[1,2]

第一作者：Jiang, Jie

通信作者：Chen, CZ[1];Sun, H[2]

机构：[1]Huaqiao Univ, Acad Adv Carbon Convers Technol, Fujian Prov Key Lab Biomass Low Carbon Convers, Xiamen 361021, Peoples R China;[2]Chinese Acad Forestry, Inst Chem Ind Forest Prod, Nanjing 210042, Peoples R China

年份：2026

卷号：204

外文期刊名：BIOMASS & BIOENERGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001588653100001)】；

基金：This research was financially supported by the National Key Research and Development Program of China (2023YFB4203701) .

语种：英文

外文关键词：Enzymatic hydrolysis lignin; Purification; Cation assisted technology; Chelation

摘要：During the pursuit of applying lignin within high value-added domains and facilitating its value-added exploitation, the problem of lignin purity emerges as a significant impediment. This study developed a novel cation-assisted strategy using metal salts (CaCl2, MgCl2, FeCl3, AlCl3) to purify enzymatic hydrolysis lignin (EHL) from pine, wheat, and corn cob. Treatment with 20 g/L salt concentration proved most effective, significantly enhancing EHL purity: pine-EHL increased from similar to 40 % to 60-85 %, wheat-EHL from similar to 40 % to 55-75 %, and corn-EHL from similar to 60 % to 75-90 %. Spectroscopic analyses (FT-IR, P-31 NMR, HSQC) confirmed a marked reduction in hydroxyl group content, indicative of chelation between metal cations and aliphatic-OH groups in EHL, which facilitated its purification. The homogeneous dispersion of metal ions within the lignin complexes was further verified by XPS and SEM. Gaussian calculations revealed that although Al3+ and Fe3+ exhibited higher binding energies (-326.09 and -102.82 kcal/mol) toward EHL compared to Ca2+ and Mg2+ (-12.51 and -24.20 kcal/mol), the divalent cations achieved superior purification performance with lignin purity exceeding 90 %. This discrepancy stems from the strong, non-selective covalent coordination of trivalent ions, which promoted cross-linking and carbohydrate co-precipitation, whereas the moderate and reversible electrostatic interactions of divalent ions enabled selective lignin precipitation without significant impurity entrapment. This research initiative, therefore, pioneers a practicable and efficacious route for the value-added utilization of purification lignin.