文献类型：期刊文献

英文题名：PdGSTF1, PdGSTU3, and PdGSTU5 are essential for the accumulation of isosalipurposide and other pigments in peonies

作者：Yuan, Meng[1] Wei, Xinrui[1] Peng, Fucheng[1] Wang, Qun[1] Zhou, Lin[1] Wang, Yan[1]

第一作者：Yuan, Meng

通信作者：Zhou, L[1];Wang, Y[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat Natl Forestry & G, Beijing 100091, Peoples R China

年份：2025

卷号：122

期号：2

外文期刊名：PLANT JOURNAL

收录：;EI(收录号：20251818355010);Scopus(收录号：2-s2.0-105004003107);WOS:【SCI-EXPANDED(收录号:WOS:001480535500009)】；

基金：This research was supported by the National Key Research and Development Program of China (Grant No. 2018YFD1000405) and the National Natural Science Foundation of China (Grant No. 31972456).

语种：英文

外文关键词：gene family; glutathione S-transferase; isosalipurposide; Paeonia delavayi var. lutea; transport

摘要：Isosalipurposide (ISP) is a critical substance that gives peony its yellow phenotype; however, studies on its synthesis and transport in petals have not yet been conducted. During plant coloration, the transport of pigments is closely related to glutathione S-transferases (GSTs). To this end, we performed the metabolomic analysis of petals from three different developmental stages of Paeonia delavayi var. lutea and combined it with transcriptomic data to comprehensively characterize the GST gene family. We identified 42 GST genes from P. delavayi var. lutea through transcriptome data mining. Among these, the molecular docking results of PdGSTF1, PdGSTU3, and PdGSTU5 showed that they have the ability to bind multiple flavonoids. Virus-induced gene silencing (VIGS) experiments and overexpression experiments demonstrated that PdGSTF1, PdGSTU3, and PdGSTU5 can not only transport ISP but also transfer various pigments, including flavone glycosides, flavonol glycosides, and anthocyanin glycosides. Additionally, through yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays, PdMYB2 was identified as a protein interacting with PdGSTF1 and PdGSTU3, co-participating in the transport of flavonoids. Our research findings have elucidated the roles of key candidate PdGSTs in pigment transportation in P. delavayi var. lutea and uncovered their underlying molecular mechanisms.