文献类型：期刊文献

英文题名：Isolation and characterization of pyrroline-5-carboxylate synthetase gene from perennial ryegrass (Lolium perenne L.)

作者：Cao, Li[1,2,3] Han, Lei[2] Zhang, Hua-Li[4,5] Xin, Hai-Bo[4,5] Imtiaz, Muhammad[3] Yi, Ming-Fang[3] Sun, Zhen-Yuan[2] Ju, Guan-Sheng[2] Qian, Yong-Qiang[2] Liu, Jun-Xiang[2]

第一作者：Cao, Li

通信作者：Sun, ZY[1]

机构：[1]Yanbian Univ, Dept Hort & Landscape, Yanji 13300, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Res Inst Forestry, Beijing 100091, Peoples R China;[3]China Agr Univ, Dept Ornamental Hort & Landscape Architecture, Beijing 100193, Peoples R China;[4]Beijing Inst Landscape Architecture, Beijing 100102, Peoples R China;[5]Beijing Key Lab Greening Plants Breeding, Beijing 100102, Peoples R China

年份：2015

卷号：37

期号：3

外文期刊名：ACTA PHYSIOLOGIAE PLANTARUM

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000350050500020)】；

基金：The work was funded by the National High Technology Research and Development Program of China (Grant No. 2011AA100209) and Jilin Provincial Department of Education (Grant No. 2013-23).

语种：英文

外文关键词：Perennial ryegrass; Pyrroline-5-carboxylate synthetase; Proline; Transgenic tobacco; Genetic engineering

摘要：Perennial ryegrass is an important turf grass and also used as a forage plant. However, abiotic stresses such as salt and drought are main limitations to its cultivation. In the present work, we cloned the gene encoding pyrroline-5-carboxylate synthetase (P5CS) from Lolium perenne, which is responsible for proline biosynthesis. This gene had a coding sequence (CDS) of 2151 base pairs (bp) encoding 716 amino acids. Multi-alignment analyses showed that the putative Lolium perenne P5CS (LpP5CS) contain all conserved functional sites and regions, and also displayed considerable similarities to Triticum astevum P5CS (TaP5CS) and Oryza sativa P5CSs (OsP5CSs). The real-time polymerase chain reaction (PCR) showed that the LpP5CS was highly expressed in leaves than in other tissues under normal conditions, and induced by sodium chloride (NaCl), abscisic acid (ABA), polyethylene glycol and cold treatments. Furthermore, ectopic expression of LpP5CS led to proline accumulation in tobacco under normal conditions. The transgenic tobacco over-expressing LpP5CS exhibited stronger tolerance to salt and drought as compared to control. These results showed, that LpP5CS responds to stress signals involving salt, drought, cold and ABA in perennial ryegrass. Our data indicate that LpP5CS might be a candidate gene for stress-associated molecular breeding in perennial ryegrass.