文献类型：期刊文献

英文题名：PagMYB216 is involved in the regulation of cellulose synthesis in Populus

作者：Wei, Kaili[1,2] Zhao, Yanqiu[1,2] Zhou, Houjun[1,2] Jiang, Cheng[1,2] Zhang, Baocai[3] Zhou, Yihua[3] Song, Xueqin[1,2,4] Lu, Mengzhu[1,2,4]

第一作者：Wei, Kaili

通信作者：Song, XQ[1];Lu, MZ[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China;[3]Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China;[4]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Jiangsu, Peoples R China

年份：2019

卷号：39

期号：5

外文期刊名：MOLECULAR BREEDING

收录：;EI(收录号：20240615512774);Scopus(收录号：2-s2.0-85064903651);WOS:【SCI-EXPANDED(收录号:WOS:000465505100001)】；

基金：This work was supported by the National Key Research and Development Program of China (2016YFD0600103) and Basic Research Fund of Research Institute of Forestry, Chinese Academy of Forestry (RIF-2013-07 and RIF-2014-08).

语种：英文

外文关键词：PagMYB216; CESA; GAMYB; Secondary wall; Cellulose synthesis; Populus

摘要：Cellulose accounts for most of the structural material in secondary cell walls. Although the synthesis of cellulose has been extensively investigated, the regulation of cellulose synthesis in woody plants remains elusive. In this study, we showed PagMYB216 to be involved in the regulation of cellulose synthesis in Populus. Subcellular localization and transcriptional activation analyses suggest that PagMYB216 is a nuclear-localized transcriptional activator. Transient transcriptional assays revealed that PagMYB216 could activate the expression of secondary cell wall cellulose synthesis genes (CESAs) through binding to the GAMYB motif. Overexpression of PagMYB216 in Populus resulted in enhanced expression of secondary wall CESAs and increased cellulose content in stems. In contrast, the dominant repression of PagMYB216 transgenic poplar plants led to the downregulation of secondary wall CESAs and decreased cellulose content. In addition, cellulose deposition and secondary wall thickness were increased in stems from PagMYB216 overexpression poplar plants and decreased in stems from PagMYB216 dominant repression poplar plants. Taken together, our results suggest that PagMYB216 is involved in the modulation of cellulose synthesis in Populus by controlling the expression of secondary wall CESAs.