文献类型：期刊文献

中文题名：测定植物全氮的H2S04-H202消煮法改进

英文题名：Improvement in the H2S04-H202Digestion Method for Determining Plant Total Nitrogen

作者：李朝英[1] 郑路[1] 卢立华[1] 李丽利[1]

第一作者：李朝英

机构：[1]中国林业科学研究院热带林业实验中心/广西友谊关森林生态系统定位观测研究站

年份：2014

卷号：30

期号：6

起止页码：159-162

中文期刊名：中国农学通报

外文期刊名：Chinese Agricultural Science Bulletin

收录：CSTPCD;;CSCD:【CSCD2013_2014】；

基金：中央级公益性科研院所基本科研业务费专项资金"基于经营班尺度的森林立地分类与评价"(CAFYBB2012001)

语种：中文

中文关键词：植物全氮;H2SO4-H2O2消煮法;改进

外文关键词：plant total nitrogen; H2S04-H202 digestion method; improvement

分类号：S-132

摘要：为了快速准确测定批量植物全氮，提高检测效率，对测定植物全氮的H2S04-H202消煮法提出改进。通过反复实验，摸索出在消煮过程只加1次H2O2的改进方法。结果表明，改进方法测定氮含量的变异系数（CV）小于5％，回收率在95％～105％之间。与原H2S04-H202消煮法相比，节约用时50-80min批样，减少H202消耗0．38～0．68mL／样，检测结果无显著性差异。改进方法的精密度及准确性符合检测分析要求，同时缩短了植物全氮检测用时，H2O2耗用量相对较少，效率明显提升，适宜批量植物全氮的检测。
In order to determinate quickly and accurately, and improve the determining efficiency, H2SO4-H202 digestion method for determining plant total nitrogen was improved. H202 was added only once in digestion process. The coefficient of variation （CV） of the improved method was less than 5%. The recoveries were in the range of 95%-105%. The time of improved method was less 50-80 rain than that of the original H2SO4-H202 digestion method. H202 consumption was saved 0.38-0.68 mL per sample. Detection results were no significant difference between the two methods. Precision and accuracy met the requirement of detection analysis. The improved method was recommended as digestion method for rapid determining batch plant total nitrogen.