文献类型：期刊文献

英文题名：Transcriptome Analysis of mRNA and miRNA in Somatic Embryos of Larix leptolepis Subjected to Hydrogen Treatment

作者：Liu, Yali[1] Han, Suying[2] Ding, Xiangming[3] Li, Xinmin[1,3] Zhang, Lifeng[1] Li, Wanfeng[1] Xu, Haiyan[1] Li, Zhexin[1] Qi, Liwang[1]

第一作者：Liu, Yali

通信作者：Qi, LW[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Key Lab Res Inst Forest Ecol & Protect, Beijing 100091, Peoples R China;[3]Univ Calif Los Angeles, Dept Pathol & Lab Med, 1000 Veteran Ave, Los Angeles, CA 90095 USA

年份：2016

卷号：17

期号：11

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000388809600178)】；

基金：This work was funded by the National Natural Science Foundation of China (Grants 31330017) and the National High Technology Research and Development Program of China (Grants 2013AA102704).

语种：英文

外文关键词：hydrogen; somatic embryogenesis; pro-embryogenic mass; miRNA; reactive oxygen species (ROS) homeostasis; cell cycle

摘要：Hydrogen is a therapeutic antioxidant that has been used extensively in clinical trials. It also acts as a bioactive molecule that can alleviate abiotic stress in plants. However, the biological effects of hydrogen in somatic embryos and the underlying molecular basis remain largely unknown. In this study, the morphological and physiological influence of exogenous H-2 treatment during somatic embryogenesis was characterized in Larix leptolepis Gordon. The results showed that exposure to hydrogen increased the proportions of active pro-embryogenic cells and normal somatic embryos. We sequenced mRNA and microRNA (miRNA) libraries to identify global transcriptome changes at different time points during H-2 treatment of larch pro-embryogenic masses (PEMs). A total of 45,393 mRNAs and 315 miRNAs were obtained. Among them, 4253 genes and 96 miRNAs were differentially expressed in the hydrogen-treated libraries compared with the control. Further, a large number of the differentially expressed mRNAs and miRNAs were related to reactive oxygen species (ROS) homeostasis and cell cycle regulation. We also identified 4399 potential target genes for 285 of the miRNAs. The differential expression data and the mRNA-miRNA interaction network described here provide new insights into the molecular mechanisms that determine the performance of PEMs exposed to H-2 during somatic embryogenesis.