文献类型：期刊文献

英文题名：An Efficient Method for Cellulose Nanofibrils Length Shearing via Environmentally Friendly Mixed Cellulase Pretreatment

作者：Chen, Yuan[1] He, Yuchan[1] Fan, Dongbin[1] Han, Yanming[1] Li, Gaiyun[1] Wang, Siqun[1,2]

第一作者：陈媛

通信作者：Li, GY[1]

机构：[1]Chinese Acad Forestry, Res Inst Wood Ind, Beijing 100091, Peoples R China;[2]Univ Tennessee, Ctr Renewable Carbon, Knoxville, TN 37996 USA

年份：2017

卷号：2017

外文期刊名：JOURNAL OF NANOMATERIALS

收录：;EI(收录号：20171503558017);Scopus(收录号：2-s2.0-85017155303);WOS:【SCI-EXPANDED(收录号:WOS:000397924700001)】；

基金：This work was supported by Special Fund for Forest Scientific Research in the Public Welfare (201504603) and National Nonprofit Special Fund for Fundamental Research from Research Institute of New Technology of Chinese Academy of Forestry (CAFINT2014K01).

语种：英文

外文关键词：Cellulose - Cellulose nanocrystals - Fourier transform infrared spectroscopy - Grinding (machining) - Materials properties - Morphology - Nanocrystalline materials - Nanofibers - Natural fibers - Scanning electron microscopy - Shearing - Surface morphology - Textile fibers - X ray diffraction

摘要：Cellulose nanofibrils (CNFs) have potential applications in the development of innovative materials and enhancement of conventional materials properties. This paper focused on the mixed cellulase hydrolysis with major activity of exoglucanase and endoglucanase on the cellulose length shearing. By the cooperation of two-step production route, including (1) enzymatic pretreatment using cellulase from Trichoderma viride and (2) mechanical grinding twice, a shorter cellulose nanofiber was fabricated. The influence of enzymatic charge and hydrolysis time on cellulose fibers was analyzed by using scanning electron microscopy (SEM), Fourier Transform Infrared Spectrometer (FTIR), and X-ray diffractometer (XRD). SEM images revealed that the surface morphology change, effective diameter sharpening, and length shearing of cellulose fibers are as a result of cellulase hydrolysis. The XRD suggested that the cellulase acted on the amorphous regions more strongly than the crystalline domains during layer-by-layer hydrolysis. The enzymatic charge and hydrolysis time significantly affected the yields and hydrolysis products concentration. The enzymatic pretreatment assisted mechanical grinding could improve the uniformity of CNF and helped to obtain CNF with exact length according to the requirement for special applications.