文献类型：期刊文献

英文题名：Neorhizobium lilium sp. nov., an endophytic bacterium isolated from Lilium pumilum bulbs in Hebei province

作者：Liu, Lei[1] Shi, Shaowen[1] Liang, Lixiong[1] Xu, Lijun[1] Chi, Miao[1,2] Zhang, Xiaoxia[3] Li, Lubin[1]

第一作者：刘蕾

通信作者：Li, LB[1];Zhang, XX[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,State Forestry A, Beijing 100091, Peoples R China;[2]Beijing Bot Garden, Beijing 100093, Peoples R China;[3]Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Key Lab Microbial Resources Collect & Preservat, Minist Agr, Beijing 100081, Peoples R China

年份：2020

卷号：202

期号：3

起止页码：609-616

外文期刊名：ARCHIVES OF MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000519159200017)】；

基金：The authors are grateful to Prof. Jianguan Sun (Key Laboratory of Microbial Resources, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences) and Prof. Junlian Gao (Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry/Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology) for their help in supporting the strain Rhizobium wenxiniae CGMCC 1.15279T.

语种：英文

外文关键词：Neorhizobium; Neorhizobium pumilum; Lilium pumilum; Endophytic bacterium

摘要：A novel gram-negative, aerobic, non-spore-forming, rod-shaped and non-nitrogen fixing bacterium named strain 24NR(T) was isolated from wild Lilium pumilum bulbs in Fuping, Baoding City, Hebei province, PR China. The 16S rRNA gene sequences of strains 24NR(T) showed the highest similarity to Neorhizobium alkalisoli DSM 21826(T) (98.5%) and N. galegae HAMBI 540(T) (98.1%). Phylogenetic analysis based on 16S rRNA genes and multilocus sequence analysis (MLSA) based on the partial sequences of atpD-glnII-glnA-recA-ropD-thrC housekeeping genes both indicated that strain 24NR(T) is a member of the genus Neorhizobium. The average nucleotide identity between the genome sequence of strain 24NR(T) and that of the isolate N. alkalisoli DSM 21826(T) was 83.1%, and the digital DNA-DNA hybridization was 20.1%. The G+C content of strain 24NR(T) was 60.3 mol %. The major cellular fatty acids were summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c) and C-19:0 cyclo omega 8c. Based on phenotypic, phylogenetic, and genotypic data, strain 24NR(T) is considered to represent a novel species of the genus Neorhizobium, for which the name Neorhizobium lilium sp. nov. is proposed. The type strain is 24NR(T) (= ACCC 61588(T) = JCM 33731(T)).