文献类型：期刊文献

英文题名：Molecular insights into lignin biosynthesis on cadmium tolerance: Morphology, transcriptome and proteome profiling in Salix matsudana

作者：Yu, Miao[1,2] Zhuo, Renying[1] Lu, Zhuchou[1] Li, Shaocui[1] Chen, Juanjuan[1] Wang, Yujun[1] Li, Jihong[3] Han, Xiaojiao[1]

第一作者：Yu, Miao

通信作者：Han, XJ[1];Li, JH[2]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding Zhejiang Prov, Hangzhou 311400, Zhejiang, Peoples R China;[2]Nanjing Forestry Univ, Forestry Fac, Nanjing 210037, Jiangsu, Peoples R China;[3]Forestry Coll Shandong Agr Univ, Tai An 271018, Shandong, Peoples R China

年份：2023

卷号：441

外文期刊名：JOURNAL OF HAZARDOUS MATERIALS

收录：;EI(收录号：20223712728154);Scopus(收录号：2-s2.0-85137628648);WOS:【SCI-EXPANDED(收录号:WOS:000874973300001)】；

基金：This work was supported by National Key Research and Develop- ment Program of China (No. 2021YFD2200201) to RZ, National Natural Science Foundation of China (No. 31872168) to XH and the Agricultural Breeding Program of Shandong Province (2021LZGC005) to JL. We thank professor Meng -Zhu Lu from Zhejiang A & F University to provide poplar calli, and Dr Hengfu Yin and Guangcai Chen from Chinese Academy of Forestry for his assistance with revising the manuscript.

语种：英文

外文关键词：Salix matsudana var; matsudana f; umbraculifera; Cd tolerance; Cell wall; Lignin biosynthesis; Transcriptome and proteome analysis

摘要：Soil pollution caused by cadmium (Cd) is a serious concern. Phytoremediation is a popular technology in the remediation of Cd-contaminated soil. Salix matsudana var. matsudana f. umbraculifera Rehd. has been charac-terized as a high Cd-accumulating and tolerant willow (HCW). Here, transcriptome and proteome profiling, along with morphology analyses were performed to explore molecular cross-talk involved in Cd tolerance. Our results showed that 73%-83% of the Cd in roots accumulated in the cell walls and root xylem cell walls were significantly thickened. From transcriptome and proteome analysis, a total of 153 up-regulated differentially -expressed genes and 655 up-regulated differentially-expressed proteins were found in common between two comparison groups (1 d and 4 d vs. respective control). Furthermore, phenylpropanoid biosynthesis was iden-tified as a key pathway in response to Cd stress. In this pathway, lignin biosynthesis genes or proteins were significantly up-regulated, and lignin content increased significantly in roots under Cd stress. Two Cd-induced genes cinnamoyl-CoA reductase 1 (SmCCR1) and cinnamyl alcohol dehydrogenase 7 (SmCAD7) from HCW increased the lignin content and enhanced Cd tolerance in transgenic poplar calli. These results lay the foun-dation for further clarifying the molecular mechanisms of Cd tolerance in woody plants.