文献类型：期刊文献

中文题名：柚木花粉收集与贮藏研究

英文题名：Collection and Storage of Tectona grandis Pollen

作者：黄桂华[1] 梁坤南[1] 林明平[1] 周再知[1] 马华明[1]

第一作者：黄桂华

机构：[1]中国林业科学研究院热带林业研究所

年份：2012

卷号：31

期号：9

起止页码：1-3

中文期刊名：种子

外文期刊名：Seed

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD_E2011_2012】；

基金："十二五"国家科技支撑课题"珍贵用材树种新品种选育技术研究"(编号:2012 BAD 01 B 05)之内容"柚木;西南桦;降香黄檀新品种选育技术研究"

语种：中文

中文关键词：柚木;花粉收集;花粉贮藏;花粉萌发

外文关键词：Tectona grandis ; pollen collection ; pollen storage ; pollen germination

分类号：S722.3

摘要：介绍了柚木开花生物学特性,开展了柚木花粉收集、活力测定、贮藏方法及其效果的研究。结果表明:柚木花粉收集可采用水培法和套袋法;不同种源收集花粉时间不同,印度种源和缅甸种源分别为09:～11:00时和11:00～13:00时;花开放后,花粉活力是先升后降,1.5 h后花粉活力最强,4.5 h后花粉即失去萌发力;随着贮藏时间的延长,5℃和-15℃干燥贮藏花粉的活力逐渐下降,贮藏到330 d,5℃和-15℃的花粉都失去萌发力;在整个过程中,5℃的花粉萌发率和花粉管长度都比-15℃下降速度快;-15℃柚木花粉的贮藏效果优于5℃;-15℃干燥贮藏240 d的柚木花粉,可满足当年柚木花期不同的种源间或柚木属不同种间人工杂交授粉。
Anthesis pheology, pollen collection, viability determination and storage of Tectona grandis L. f. were introduced and studied, the results showed that pollen can be collected by water culture method and bagged method, the time for pollen collection of Indian provenances and Burma provenances were 9 to 11 o-clock and 11 to 13 o-clock,respectively. The pollen viability rose first and then decreased after anthesis,it have highest viability after anthesis 1.5 h and no longer viable after 4.5 h. The pollen viability decreased fast after collected and put in room temperature for wind to dry, it no longer viable after collected 2 h. The viability of pollen stored in 5 ~C and - 15 ~C rose first and then decreased, both of the pollen lost viability at 330 day. Both pollen germination rate and pollen tube length of pollen stored in 5 ℃ decreased fast than that of - 15 ℃, pollen storage effect of - 15 ℃ was better than 5 ℃. Pollen stored in - 15 ℃ for 240 day can be used for controlled pollination among different teak provenances or different species of Tectona.