文献类型：期刊文献

英文题名：Populus euphratica Apyrases Increase Drought Tolerance by Modulating Stomatal Aperture in Arabidopsis

作者：Zhang, Yanli[1] Sun, Yuanling[1] Liu, Xiaojing[1] Deng, Jiayin[1] Yao, Jun[1] Zhang, Yinan[2] Deng, Shurong[3] Zhang, Huilong[4] Zhao, Nan[1] Li, Jinke[1] Zhou, Xiaoyang[1] Zhao, Rui[1] Chen, Shaoliang[1]

第一作者：Zhang, Yanli

通信作者：Chen, SL[1]

机构：[1]Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Beijing 100083, Peoples R China;[2]Chinese Acad Forestry, Forestry Inst New Technol, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[4]Chinese Acad Forestry, Res Ctr Saline & Alkali Land, Natl Forestry & Grassland Adm, Beijing 100091, Peoples R China

年份：2021

卷号：22

期号：18

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000700780300001)】；

基金：This research was funded by the National Natural Science Foundation of China, grant numbers 32071730, 31770643 and 31570587, the Beijing Municipal Natural Science Foundation, grant numbers 6182030 and 6172024, and the Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Municipal Education Commission.

语种：英文

外文关键词：poplar; extracellular ATP; apyrase 1; apyrase 2; abscisic acid; stomatal aperture; water stress; water loss; light; guard cell

摘要：Stomatal regulation is crucial to reduce water consumption under drought conditions. Extracellular ATP (eATP) serves as a signaling agent in stomatal regulation; however, it is less known whether the eATP mediation of stomatal aperture is linked to apyrases (APYs), the principal enzymes that control the concentration of eATP. To clarify the role of APYs in stomatal control, PeAPY1 and PeAPY2 were isolated from Populus euphratica and transferred into Arabidopsis. Compared with the wild-type Arabidopsis and loss-of-function mutants (Atapy1 and Atapy2), PeAPY1- and PeAPY2-transgenic plants decreased stomatal aperture under mannitol treatment (200 mM, 2 h) and reduced water loss during air exposure (90 min). The role of apyrase in stomatal regulation resulted from its control in eATP-regulated stomatal movements and increased stomatal sensitivity to ABA. The bi-phasic dose-responses to applied nucleotides, i.e., the low ATP (0.3-1.0 mM)-promoted opening and high ATP (>2.0 mM)-promoted closure, were both restricted by P. euphratica apyrases. It is noteworthy that eATP at a low concentration (0.3 mM) counteracted ABA action in the regulation of stomatal aperture, while overexpression of PeAPY1 or PeAPY2 effectively diminished eATP promotion in opening, and consequently enhanced ABA action in closure. We postulate a speculative model of apyrase signaling in eATP- and ABA-regulated stomatal movements under drought.