文献类型：期刊文献

中文题名：千年桐物种特异PGK3-TPS10-CYP726基因簇挖掘及其进化和表达特征

英文题名：Identication of Vernicia montana species-specific PGK3-TPS10-CYP726 gene cluster and its evolution and expression characteristics

作者：王银行[1,2] 高暝[2] 赵耘霄[2] 汪阳东[2] 李伟[1] 陈益存[2]

第一作者：王银行

机构：[1]林木遗传育种国家重点实验室,东北林业大学,黑龙江哈尔滨150040;[2]中国林业科学研究院亚热带林业研究所,浙江杭州311400

年份：2025

卷号：47

期号：2

起止页码：1-9

中文期刊名：北京林业大学学报

外文期刊名：Journal of Beijing Forestry University

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金面上项目(31971685);浙江省科技创新领军人才项目(2022R52028)。

语种：中文

中文关键词：千年桐;基因簇;物种特异;进化起源;细胞色素P450;磷酸甘油酸激酶3;抗枯萎病

外文关键词：Vernicia montana;gene cluster;species-specific;evolutionary origin;CYP450;PGK3;resistance to Fusarium wilt

分类号：S794.3

摘要：【目的】基于全基因组挖掘高质量物种特异基因簇,为深入分析物种性状和遗传改良提供新的思路。【方法】通过基因组、转录组、蛋白数据联合分析,利用生物信息手段进行高质量基因簇预测与筛选,对目标基因簇进行基因组共线性、基因组结构、系统进化和转录表达分析。【结果】(1)预测了千年桐高可信度PGK3-TPS10-CYP726基因簇,该基因簇中共包括13个酶编码基因,具体包括:3-磷酸甘油醛脱氢酶3(GAPCP3)、CHD4细胞色素P45071D(CYP71D55)、细胞色素P450726A(CYP726A)、萜类合酶10(TPS10)、磷酸甘油酸激酶3(PGK3)等。(2)PGK3-TPS10-CYP726基因簇具有良好的共表达和共通路特征,在千年桐基因组中展示了良好的独特性和完整性。(3)PGK3-TPS10-CYP726基因簇有约一半基因在双子叶植物中存在较高的保守性,但在单子叶植物中存在基因缺失的现象;PGK3基因一直保留于单双子叶植物和苔藓植物中,其序列在进化过程中高度保守;推测PGK3-TPS10-CYP726基因簇起源于早期基部类群,并在千年桐物种分化后形成。(4)千年桐物种特异PGK3-TPS10-CYP726基因簇在根部特异高表达,在枯萎病病原菌侵染后的早、中期的千年桐根木质部呈现持续显著高表达。【结论】首次鉴定了千年桐物种特异基因簇PGK3-TPS10-CYP726,并分析了其进化起源和表达特征,为解析千年桐抗枯萎病等特有性状提供了新的视角。
[Objective] Identification of high-quality species-specific gene clusters at the genome-wide level provides new ideas for in-depth analysis of species traits and genetic improvement.[Method] By combining genomic,transcriptome,and protein data analysis,high-quality gene cluster prediction and screening were carried out using bioinformatics methods.Genomic collinearity,genomic structure,systematic evolution,and transcriptional expression analysis were performed on target gene clusters.[Result](1) The PGK3-TPS10-CYP726 gene cluster with high confidence was predicted,including 13enzyme coding genes,i.e.glyceraldehyde 3-phosphate dehydrogenase 3(GAPCP3),CHD4 cytochrome P45071D(CYP71D55),cytochrome P450726A(CYP726A),terpenoid synthase 10(TPS10),phosphoglycerate kinase 3(PGK3),etc.(2) PGK3-TPS10-CYP726 gene cluster had good co-expression and common pathway characteristics,and showed good uniqueness and integrity in genome of Vernicia montana.(3) About half of PGK3-TPS10-CYP726 gene cluster was highly conserved in dicotyledonous plants,but it was absent in monocotyledonous plants.The PGK3 gene had been preserved in monodicotyledonous plants and bryophytes,and its sequence was highly conserved during evolutionary process.It was speculated that the PGK3-TPS10-CYP726 gene cluster originated from the early basal taxa and formed after V.montana species differentiation.(4) The specific PGK3-TPS10-CYP726 gene cluster of V.montana species was specifically highly expressed in roots,and the xylem of root showed a sustained and significant high expression in the early and middle stages after being infected by Fusarium wilt pathogen.[Conclusion] The specific gene cluster PGK3-TPS10-CYP726 is identified for the first time,and its evolutionary origin and expression characteristics are analyzed,which provides a new perspective for analyzing the unique traits of V.montana such as its resistance to Fusarium wilt.