文献类型：期刊文献

英文题名：MicroRNA comparison between poplar and larch provides insight into the different mechanism of wood formation

作者：Li, Hui[1,2] Huang, Xiong[2,3] Li, Wanfeng[3] Lu, Yan[2,3] Dai, Xinren[2] Zhou, Zaizhi[1] Li, Quanzi[2,3]

通信作者：Zhou, ZZ[1];Dai, XR[2]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China

年份：2020

卷号：39

期号：9

起止页码：1199-1217

外文期刊名：PLANT CELL REPORTS

收录：;Scopus(收录号：2-s2.0-85086781196);WOS:【SCI-EXPANDED(收录号:WOS:000542514800001)】；

基金：This work was supported by Grant CAFYBB2017ZY001 from Fundamental Research Funds of the Chinese Academy of Forestry.

语种：英文

外文关键词：microRNAs; Conservation analysis; GO analysis; KEGG analysis; Gymnosperm; Angiosperm

摘要：Key message MiRNA transcriptome analysis of different tissues in poplar and larch suggests variant roles of miRNAs in regulating wood formation between two kinds of phyla. Poplar and larch belong to two different phyla. Both are ecological woody species and major resources for wood-related industrial applications. However, wood properties are different between these two species and the molecular basis is largely unknown. In this study, we performed high-throughput sequencing of microRNAs (miRNAs) in the three tissues, xylem, phloem and leaf ofPopulus alba x Populus glandulosaandLarix kaempferi. Differentially expressed miRNA (DEmiRNA) analysis identified 85 xylem-specific miRNAs inP. alba x P. glandulosaand 158 xylem-specific miRNAs inL. kaempferi.Among 36 common miRNAs, 12 were conserved between the two species. GO and KEGG analyses of the miRNA target genes showed similar metabolism in two species. Through KEGG and BLASTN, we predicted target genes of xylem differentially expressed (DEmiRNA) in the wood formation-related pathways and located DEmiRNAs in these pathways. A network was built for wood formation-related DEmiRNAs, their target genes and orthologous genes inArabidopsis thaliana. Comparison of DEmiRNA and target gene annotation betweenP. alba x P. glandulosaandL. kaempferisuggested the different functions of DEmiRNAs and divergent mechanism in wood formation between two species, providing knowledge to understand wood formation mechanism in gymnosperm and angiosperm woody plants.