文献类型：期刊文献

英文题名：DEVELOPMENT OF 198 NOVEL EST-DERIVED MICROSATELLITES IN EUCALYPTUS (MYRTACEAE)

作者：He, Xudong[1,2,3] Wang, Yu[1,2,3] Li, Fagen[2,3] Weng, Qijie[2,3] Li, Mei[2,3] Xu, Li-an[1] Shi, Jisen[1,2] Gan, Siming[2,3]

第一作者：He, Xudong

通信作者：Shi, JS[1]

机构：[1]Nanjing Forestry Univ, Key Lab Forest Genet & Biotechnol, Minist Educ, Nanjing 210037, Jiangsu, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China

年份：2012

卷号：99

期号：4

起止页码：E134-E148

外文期刊名：AMERICAN JOURNAL OF BOTANY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000302810400002)】；

基金：The authors thank Kunming Wu and Juying Wu for plant material maintenance as well as Ye Sun and Xiaoli Yu for technical assistance. This work was supported by the Ministry of Science and Technology of China (2012CB114505 and 2011AA100202), the National Natural Science Foundation of China (31070592), and the Ministry of Finance of China through a specific program for national nonprofit scientific institutions (RITFKYYW2010-04).

语种：英文

外文关键词：Eucalyptus; expressed sequence tags-simple sequence repeat markers; Myrtaceae; polymorphism

摘要：Premise of the study: Expressed sequence tag (EST)-derived microsatellites were identified in Eucalyptus through screening the GenBank database. The loci were sequence-verified and explored for polymorphism among 20 genotypes. Methods and Results: In total, 198 novel microsatellites were developed from 8262 unigenes, with the identity of 73.6-100% to the original sequences and presence of the expected repeat motifs. One hundred and eighty-four markers proved to be polymorphic among 10 E. urophylla and 10 E. tereticornis genotypes, with the number of alleles per locus, observed heterozygosity, and polymorphic information content being 2-17 (mean: 7.11), 0-1.0 (mean: 0.4511), and 0.0940-0.9131 (mean: 0.6571), respectively. Conclusions: These markers will be useful for germplasm characterization, genome mapping, and gene tagging for economic traits in the two species examined and may have potential for genetic applications in Eucalyptus