文献类型：期刊文献

英文题名：Identification and Validation of Reference Genes for Gene Expression Analysis in Schima superba

作者：Yang, Zhongyi[1,2,3] Zhang, Rui[1,3] Zhou, Zhichun[1,3]

第一作者：Yang, Zhongyi

通信作者：Zhang, R[1];Zhang, R[2]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[2]Nanjing Forestry Univ, Coll Landscape Architecture, Nanjing 210037, Peoples R China;[3]Zhejiang Prov Key Lab Tree Breeding, Hangzhou 311400, Peoples R China

年份：2021

卷号：12

期号：5

外文期刊名：GENES

收录：;Scopus(收录号：2-s2.0-85106903599);WOS:【SCI-EXPANDED(收录号:WOS:000653947500001)】；

基金：This study was supported by the Zhejiang Provincial Natural Science Foundation of China (grant number LQ19C160002), the Special Fund for Breeding New Varieties of Forest Species in Zhejiang Province (grant number 2016C020563), the Science and Technology Innovation Project in Jiangxi Province (grant number (2019)19), and the Sixth Stages of Planting and Seedling Science and Technology of Fujian Province (grant number 201906).

语种：英文

外文关键词：reference gene; real-time quantitative PCR; Schima superba; tissues; stability evaluation

摘要：Real-time quantitative PCR (RT-qPCR) is a reliable and high-throughput technique for gene expression studies, but its accuracy depends on the expression stability of reference genes. Schima superba is a fast-growing timber species with strong resistance. However, thus far, reliable reference gene identifications have not been reported in S. superba. In this study, 19 candidate reference genes were selected and evaluated for their expression stability in different tissues of S. superba. Three software programs (geNorm, NormFinder, and BestKeeper) were used to evaluate the reference gene transcript stabilities, and comprehensive stability ranking was generated by the geometric mean method. Our results show that SsuACT was the most stable reference gene and that SsuACT + SsuRIB was the best reference gene combination for different tissues. Finally, the stable and less stable reference genes were verified using SsuSND1 expression in different tissues. To our knowledge, this is the first report to verify appropriate reference genes for normalizing gene expression in S. superba for different tissues, which will facilitate the future elucidation of gene regulations in this species and useful references for relative species.