文献类型：期刊文献

英文题名：A natural biopreservative: Antibacterial action and mechanisms of Chinese Litsea mollis Hemsl. extract against Escherichia coli DH5 alpha and Salmonella spp.

作者：Cai, XiaoTian[1] Wang, Xue[2,3] Chen, YiCun[2,3] Wang, YangDong[2,3] Song, DaFeng[1] Gu, Qing[1]

第一作者：Cai, XiaoTian

通信作者：Song, DF[1];Gu, Q[1]

机构：[1]Zhejiang Gongshang Univ, Key Lab Food Microbial Technol Zhejiang Prov, Hangzhou 310018, Zhejiang, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China

年份：2019

卷号：102

期号：11

起止页码：9663-9673

外文期刊名：JOURNAL OF DAIRY SCIENCE

收录：;Scopus(收录号：2-s2.0-85070881795);WOS:【SCI-EXPANDED(收录号:WOS:000489285000010)】；

基金：This research was funded by the Natural Science Foundation of Zhejiang Province (No. LY18C06004, Hangzhou, China) and the National Natural Science Foundation of China (Beijing; No. 31871775).

语种：英文

外文关键词：antibacterial activity; Litsea mollis Hemsl. essential oil; mechanism of action; chemical composition

摘要：Chemical preservatives have potential safety hazards, which may pose threats to human health. Safer biopreservatives are therefore urgently required. This study investigated the bacteriostatic activity arid mechanism of Litsea mollis Hemsl. essential oil against Escherichia coli DH5 alpha and Salmonella spp. Antibacterial activity of Litsea mollis Hemsl. essential oil 9 (LMEO9) against E. coli DH5 alpha was observed (zone of inhibition was 5.0 +/- 0.2 mm; minimum inhibitory concentration was 0.05%). Increases in electrolyte, nucleic acid, and alkaline phosphatase leakage in LMEO9-treated bacteria suggested that the cell envelope had been damaged. Scanning and transmission electron microscopy also demonstrated morphological alterations and content leakage during LMEO9 treatment. According to the kill-time analysis arid propidium iodide uptake assay, LMEO9 led to cell death. These results demonstrated that LMEO9, which could affect bacterial cell envelope structural integrity, is a low-cost biopreservative that could be useful for the dairy industry and in fresh storage.