文献类型：期刊文献

中文题名：胡杨F-box基因克隆和功能分析

英文题名：Identification and Functional Analysis of F-box Gene from Populus Euphratica

作者：任逸秋[1,2] 贾会霞[2] 郭英华[2] 徐向东[2] 李建波[2] 卢孟柱[2] 樊军锋[1] 胡建军[2]

第一作者：任逸秋

机构：[1]西北农林科技大学林学院,杨凌712100;[2]林木遗传育种国家重点实验室,国家林业局林木培育重点实验室,中国林业科学研究院林业研究所,北京100091

年份：2017

卷号：15

期号：5

起止页码：1655-1662

中文期刊名：分子植物育种

外文期刊名：Molecular Plant Breeding

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：中央级公益性科研院所基本科研业务费专项资金(CAFYBB2014ZX001-4);林业公益性行业科研专项经费(201404101);新疆自治区高层次人才引进工程(2014)共同资助

语种：中文

中文关键词：F-box;亚细胞定位;表达特性;原生质体转化;耐盐性

外文关键词：F-box, Expression characteristic, Subcellular localization, Protoplast transformation, Salt-resistance

分类号：S

摘要：F-box蛋白在泛素-蛋白酶体途径中能够特异识别底物蛋白,调节多种激素的信号转导途径,并响应多种非生物胁迫,参与植物抗逆途径。胡杨(Populus euphratica)是中国西北沙漠唯一能成林的高大乔木树种,对盐碱、干旱、极端温度等抵抗能力较强,是研究林木抗逆机理的候选模式树种。本研究对胡杨耐盐性状关联分析获得的F-box基因进行克隆、表达特性分析、亚细胞定位和转化胡杨原生质体的耐盐性测定,旨在研究胡杨F-box基因的功能特性。胡杨F-box基因全长1 086 bp,编码361个氨基酸,含有一个F-box domain和一个F-box associated domain。q RT-PCR表明胡杨F-box基因在各组织中均有表达,无组织特异性,其表达受盐、高温、低温、干旱和ABA胁迫诱导。PEG介导的胡杨原生质体瞬时表达发现F-box蛋白定位于细胞核上,流式细胞术证实过表达F-box基因能够提高胡杨原生质体的耐盐性。研究初步揭示了胡杨F-box基因的表达特性和耐盐性,为进一步解析胡杨F-box基因的功能及胡杨抗逆分子机理提供帮助。
F-box protein could specifically identify the substrate protein in the ubiquitin-proteasome pathway and regulate the signal transduction pathways of hormones. Moreover, F-box gene responses to a variety of abiotic stresses and involves in stress-tolerant pathway in plants. Populus euphratica, a only forest species in the desert region of northwest China, has a strong resistance to salt, drought, extreme temperatures, etc. Thus, it had been considered as a candidate model tree species for the study of mechanism of stress resistance. In this study, the gene cloning, expression characteristics analysis, subcellular localization and salt resistance analysis of F-box gene that obtained by associated analysis for salt-tolerance traits of P. euphratica were performed to search its function characteristics. F-box gene ofP. euphratica contained 1 086 nucleotides, which coded 361 amino acids containing F-box domain and F-box associated domain. The results of qRT-PCR showed that F-box gene was expressed in various tissues, without tissue specificity. Its expression was induced by the salt, high temperature, low temperature,drought, and ABA stresses. Transient expressing ofP. euphratica protoplast mediated by PEG showed F-box protein was located in nucleus, and it enhanced the salt resistance ofprotoplast. The preliminary results of this study revealed the expression characteristics and salt resistance of F-box gene from P. euphratica, and provided a reference for further analysis of F-box gene function and molecular mechanism with resistance to stress in P. euphratica.