文献类型：期刊文献

英文题名：Evolutionary Divergence of Duplicated Hsf Genes in Populus

作者：Liu, Bobin[1] Hu, Jianjun[2] Zhang, Jin[2,3]

第一作者：Liu, Bobin

通信作者：Zhang, J[1];Zhang, J[2]

机构：[1]Fujian Agr & Forestry Univ, Fujian Coll & Univ Engn Res Inst Conservat & Util, Coll Forestry, Fuzhou 350002, Fujian, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,Natl Forestry &, Beijing 100091, Peoples R China;[3]Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37831 USA

年份：2019

卷号：8

期号：5

外文期刊名：CELLS

收录：;Scopus(收录号：2-s2.0-85074167110);WOS:【SCI-EXPANDED(收录号:WOS:000470994400058)】；

基金：This study was funded by the Forestry Peak Discipline Construction Project (71201800721) and National Natural Science Foundation of China (31870661) to B.L., National Nonprofit Institute Research Grant of the Chinese Academy of Forestry (CAFYBB2017ZY008) and (CAFYBB2018ZY001-9) to J.H.

语种：英文

外文关键词：Populus; heat shock transcription factors; gene duplication; gene expression; alternative splicing; single nucleotide polymorphism; protein structure; co-expression network

摘要：Heat shock transcription factors (Hsfs), which function as the activator of heat shock proteins (Hsps), play multiple roles in response to environmental stress and the development of plants. The Hsf family had experienced gene expansion via whole-genome duplication from a single cell algae to higher plants. However, how the Hsf gene family went through evolutionary divergence after genome duplication is unknown. As a model wood species, Populus trichocarpa is widely distributed in North America with various ecological and climatic environments. In this study, we used P. trichocarpa as materials and identified the expression divergence of the PtHsf gene family in developmental processes, such as dormant bud formation and opening, catkins development, and in response to environments. Through the co-expression network, we further discovered the divergent co-expressed genes that related to the functional divergence of PtHsfs. Then, we studied the alternative splicing events, single nucleotide polymorphism distribution and tertiary structures of members of the PtHsf gene family. In addition to expression divergence, we uncovered the evolutionary divergence in the protein level which may be important to new function formations and for survival in changing environments. This study comprehensively analyzed the evolutionary divergence of a member of the PtHsf gene family after genome duplication, paving the way for further gene function analysis and genetic engineering.