文献类型：期刊文献

英文题名：PaMYB11 promotes suberin deposition in Norway spruce embryogenic tissue during cryopreservation: A novel resistance mechanism against osmosis

作者：Hu, Jiwen[1] Zhu, Tianqing[2] Yao, Chengcheng[1] Hao, Chunhui[1] Yan, Huiling[1] Pu, Ziyan[1] Ma, Wenjun[1] Gao, Benwang[3] Gao, Han[3] Kong, Lisheng[4] Zhang, Hanguo[5] Wang, Junhui[1,2]

第一作者：Hu, Jiwen

通信作者：Wang, JH[1];Zhu, TQ[2];Wang, JH[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, State Forestry Adm,Res Inst Forestry, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Three Gorges Bot Garden, Management Off, Yichang 443111, Hubei, Peoples R China;[4]Univ Victoria, Ctr Forest Biol, Dept Biol, Victoria, BC V8P 5C2, Canada;[5]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China

年份：2024

卷号：119

期号：5

起止页码：2199-2216

外文期刊名：PLANT JOURNAL

收录：;EI(收录号：20242916709018);Scopus(收录号：2-s2.0-85198376509);WOS:【SCI-EXPANDED(收录号:WOS:001266112700001)】；

基金：The Norway spruce (Picea abies [L.] Karst.) embryogenic culture cell line PaVIII was kindly provided by Dr. Armand Seguin. In addition, we thank Dr. Yuan Cao from the National Key Laboratory of Chinese Academy of Forestry for her excellent technical assistance with the morphology investigation (TEM observation). This work was financially supported by the National Key Research and Development Program of China (2023YFD2200603), the Fundamental Research Funds for the Central Nonprofit Research Institution of CAF (CAFYBB2023QD001) and Postdoctoral Fellowship Program of CPSF (GZC20233017). Many thanks go to Bluescape Hebei Biotech Co. Ltd. (Baoding P.R. China) and the faculty participating in this study. Special thanks also go to the anonymous referees for their constructive comments on the study, which have greatly improved its presentation.

语种：英文

外文关键词：Picea abies; osmosis; suberin; MYB; embryogenic tissue; cryopreservation; RNA-seq

摘要：The osmotic resistance mechanism has been extensively studied in whole plants or plant tissues. However, little is known about it in embryogenic tissue (ET) which is widely used in plant-based biotechnological systems. Suberin, a cell wall aliphatic and aromatic heteropolymer, plays a critical role in plant cells against osmosis stress. The suberin regulatory biosynthesis has rarely been studied in gymnosperms. Here, PaMYB11, a subgroup 11 R2R3-MYB transcription factor, plays a key role in the osmotic resistance of Norway spruce (Picea abies) ETs during cryoprotectant pretreatment. Thus, RNA-seq, histological, and analytical chemical analyses are performed on the stable transformations of PaMYB11-OE and PaMYB11-SRDX in Norway spruce ETs. DAP-seq, Y1H, and LUC are further combined to explore the PaMYB11 targets. Activation of PaMYB11 is necessary and sufficient for suberin lamellae deposition on Norway spruce embryogenic cell walls, which plays a decisive role in ET survival under osmotic stress. Transcriptome analysis shows that PaMYB11 enhances suberin lamellae monomer synthesis by promoting very long-chain fatty acid (VLCFA) synthesis. PaPOP, PaADH1, and PaTET8L, the first two (PaADH1 and PaPOP, included) involved in VLCFA synthesis, are proved to be the direct targets of PaMYB11. Our study identified a novel osmotic response directed by PaMYB11 in Norway spruce ET, which provides a new understanding of the resistance mechanism against osmosis in gymnosperms.