文献类型：期刊文献

英文题名：Improving tobacco freezing tolerance by co-transfer of stress-inducible CbCBF and CbICE53 genes

作者：Lin, P.[1] Shen, C.[2] Chen, H.[2] Yao, X. H.[1] Lin, J.[2]

第一作者：林萍

通信作者：Yao, XH[1];Lin, J[2]|[a00051e90d3009f1c7c75]姚小华;

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Fuyang 311400, Zhejiang, Peoples R China;[2]Fudan Univ, Inst Plant Biol, Sch Life Sci, Shanghai 200438, Peoples R China

年份：2017

卷号：61

期号：3

起止页码：520-528

外文期刊名：BIOLOGIA PLANTARUM

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000405279000013)】；

基金：We are grateful for the financial support from the Natural Science Foundation of China (31370346), the National Key Technology R&D Program (2009BADA8B04), the National High Technology Research and Development Program of China (2008AA10Z105). The first two authors contributed equally to this work.

语种：英文

外文关键词：Capsella bursa-pastoris; cold inducible genes; Nicotiana tabaccum; stress inducible promoter; transgenic plants

摘要：Cold stress is one of the major limitations to crop productivity worldwide. We investigated the effects of multiple gene expression from cold tolerant Capsella bursa-pastoris in transgenic tobacco (Nicotiana tabaccum) plants. We combined CblCE53 and CbCBF into a reconstruct vector by isocaudomers. Plant overexpression of CbICE53 under the stress inducible CbCOR15b promoter and CbCBF under a constitutive promoter showed increased tolerance to both chilling and freezing temperatures in comparison to wild-type plants, according to the electrolyte leakage and relative water content. The expressions of endogenous cold-responsive genes in transgenic tobacco (NtDREB1, NtDREB3, NtERD10a and NtERD10b) were obviously upregulated under normal and low temperature conditions. These results suggest that the CbICE53 + CbCBF transgenic plants showed a much greater cold tolerance as well as no dwarfism and delayed flowering. Thus they can be considered as a potential candidate for transgenic engineering for cold tolerant tobacco.