文献类型：期刊文献

英文题名：Sustainable protection of multiple hosts against polyphagous pests using Plant Probiotic-Based Gene Silencing

作者：Chen, Jing[1] Zhang, Rong[1] Zhang, Xun[1,2] Li, Chengjin[1] Yang, Jie[1] Kong, Xiangbo[1] Liu, Fu[1] Fang, Jiaxing[1] Zhang, Sufang[1]

第一作者：Chen, Jing

机构：[1] Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing, 100091, China; [2] Airport Research Institute, China Academy of Civil Aviation Science and Technology, Beijing, 100028, China

年份：2025

外文期刊名：Trends in Biotechnology

收录：EI(收录号：20253719139927);Scopus(收录号：2-s2.0-105015566623)

语种：英文

外文关键词：Environmental protection - Genes - Metabolism - Physiology - Plant diseases - Plants (botany) - Population statistics - Probiotics - RNA

摘要：RNAi is a gene-silencing mechanism mediated by double-stranded RNA (dsRNA) molecules and is a promising pest control technology. However, challenges, such as limited persistence or narrow plant protection spectra in current RNAi application strategies, hinder its effectiveness against polyphagous pests. Here, we report an approach using plant probiotics to express dsRNA, propelling sustainable protection of multiple host plants. A Bacillus strain isolated from host plants of the polyphagous pest Hyphantria cunea, was engineered to express dsRNA targeting the pest. The modified SH-F8 strain significantly reduced H. cunea pupation rates and increased mortality by disrupting both energy metabolism and cuticle formation. The engineered SH-F8 strain exhibited successful colonization in two host plant species of H. cunea under field conditions, with enhanced population densities appearing under high-temperature/high-humidity conditions. This approach, termed ‘Plant Probiotic-Based Gene Silencing’ (PPGS), may offer a sustainable solution for multi-plant protection against polyphagous pests. ? 2025 Elsevier Ltd