文献类型：期刊文献

英文题名：Genome-wide analysis of superoxide dismutase genes in Larix kaempferi

作者：Han, Xue-Min[1,2] Chen, Qiang-Xin[2] Yang, Qi[2] Zeng, Qing-Yin[1,2] Lan, Ting[3] Liu, Yan-Jing[1]

第一作者：韩学敏;Han, Xue-Min

通信作者：Liu, YJ[1];Lan, T[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China;[3]Shenzhen Univ, Coll Life Sci & Oceanog, Guangdong Prov Key Lab Plant Epigenet, Shenzhen 518060, Peoples R China

年份：2019

卷号：686

起止页码：29-36

外文期刊名：GENE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000456755500005)】；

基金：This study was supported by the Forestry Industry Research Special Funds for Public Welfare Projects (201504104) and the National Science Fund for Distinguished Young Scholars of China (31425006).

语种：英文

外文关键词：Gene expression; Subcellular localization; Biochemical properties; Salt tolerance; Functional divergence

摘要：Superoxide dismutase is a key enzyme that scavenges superoxide anion and plays vital roles in plant antioxidant system. This study identified six SOD genes from the deciduous conifer Larix kaempferi, which is widely distributed across the cooler regions of the northern hemisphere. These six SOD genes were classified into three types: Cu/Zn-SOD (LkSOD1, 2, 3 and 4), Fe-SOD (LkSOD5) and Mn-SODs (LkSOD6). Three Cu/Zn-SOD proteins (LkSOD1, 3 and 4) were cytosolic-localized, while the other three proteins (LkSOD2, 5 and 6) were chloroplast-localized. Larix SOD proteins displayed catalytic activities toward superoxide anion, and retained > 55% of its maximum enzymatic activity between 10 degrees C and 40 degrees C. Over expressions of three Larix SOD genes (LkSOD2, 4 and 6) in Arabidopsis thaliana, respectively, showed increased germination rates and root lengths during salt stress. LkSOD5 gene could rescue pale green and dwarf phenotype of Arabidopsis atfsd2-2 mutant. Taken together, this study provided comprehensive insight into the gymnosperm SOD gene family.