文献类型：期刊文献

中文题名：葡萄籽原花青素对秀丽隐杆线虫抗衰老的影响

英文题名：Potential anti-aging effects of grape seed procyanidins on Caenorhabditis elegans

作者：王艳菊[1] 马建伟[2] 王曦茁[2] 周方[1] 张柏林[1] 汪来发[2]

第一作者：王艳菊

机构：[1]北京林业大学生物科学与技术学院,北京100083;[2]中国林业科学研究院森林生态环境与保护研究所,国家林业局森林保护学重点实验室,北京100091

年份：2014

卷号：35

期号：20

起止页码：369-373

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：林业公益性行业科研专项资助项目(200904014-3)

语种：中文

中文关键词：衰老;原花青素;秀丽隐杆线虫

外文关键词：aging ; procyanidins ; Caenorhabditis elegans

分类号：TS201.4

摘要：以秀丽隐杆线虫作为模型,研究了葡萄籽原花青素(grape seed procyanidins,GSP)对线虫寿命、生殖能力和运动能力的影响,进行了热应激和氧化应激实验,探讨了原花青素影响线虫衰老的方式。结果表明:当原花青素浓度分别为100、200、300μg/mL时,与对照组相比,采用200μg/mL原花青素喂养秀丽隐杆线虫,线虫的平均寿命延长了25.7%(p<0.01),最大寿命延长了24.0%(p<0.01),线虫的运动能力增强,平均每天每分钟爬过的峰数延长了13.2%(p<0.05),可以作为喂养秀丽隐杆线虫的最佳剂量。与对照组相比,采用200μg/mL原花青素饲喂线虫,线虫的产卵高峰期延迟,但对产卵量总数和孵化率无显著影响;热应激胁迫中,线虫的平均生存时间显著延长了27.3%(p<0.01);氧化应激胁迫中,线虫的平均生存时间延长了11.2%(p<0.05)。结论:饲喂葡萄籽原花青素可以改善线虫的平均寿命,且主要是通过提高线虫的压力应激能力来延缓秀丽隐杆线虫的衰老。
Caenorhabditis elegans was used as a lifespan model to evaluate the anti-aging potential of grape seed procyanidins（GSP）. The tolerance of C. elegans and paraquat-induced oxidization in NGM medium containing GSP（ 100,200,300μg/mL, respectively） were tested. Compared with the controls,feeding of 200μg. mL-1 GSP to C. elegans improved significantly the life span of the nematodes. The mean lifespan of C. elegans was lengthened by 25.7% （p 〈0.01）,and its maximum lifespan increased by 24.0% （p〈0.01）. In addition,the nematodes showed a good movement ability,and their average daily number of peaks per minute were prolonged significantly by 13.2%（p〈0.05）. Compared to the controls,feeding nematodes of 200μg/mL GSP to C. elegans caused the nematode＇s fecundity to postpone 1 day,although their total mean fecundity and hatchability had no significant differences from those of the controls. In the presence of 20μg/mL GSP,the mean survival time of C. elegans was lifted by 27.3% for thermotolerance（p〈0.01 ） and 11.2%（p〈0.05） for paraquat-induced oxidization,respectively. Apparently,consumption of grape seed procyanidins prolonged the average lifespan of the nematodes mainly because C. elegans improved its tolerance towards stress conditions which caused aging.