文献类型：期刊文献

中文题名：柚木愈伤组织的LBA 4404转化条件

英文题名：Genetic Transformation of Teak Callus Mediated by Agrobacterium tumefeciens Strain LBA4404

作者：曾炳山[1] 裘珍飞[1] 李湘阳[1] 刘英[1] 梁坤南[1]

第一作者：曾炳山

机构：[1]中国林业科学研究院热带林业研究所

年份：2008

卷号：28

期号：3

起止页码：60-64

中文期刊名：中南林业科技大学学报

外文期刊名：Journal of Central South University of Forestry & Technology

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD_E2011_2012】；

基金：中国林科院技术开发基金重点项目"柚木嫩枝扦插及繁育技术研究"

语种：中文

中文关键词：生物技术;柚木;农杆菌;愈伤组织;遗传转化

外文关键词：biotechnology; Tectona grandis; Agrobacterium tumeficiens; callus; genetic transformation

分类号：Q943.1

摘要：对农杆菌LBA4404转化柚木愈伤组织的适宜条件进行了优化．结果表明；LBA4404转化柚木愈伤组织的适宜预培养时间为11～15d，菌液光密度为0．5～0．8，共培养时间为13d，共培养的pH为5．6～6．2；重新悬浮的作用明显，乙酰丁香酮的作用不明显；不同无性系对LBA4404侵染的敏感性不一样，选择适宜的无性系可显著提高转化效率．
This paper deals with the genetic transformation of teak callus mediated by agro bacterium strain LBA4404. The results show that the optimized pre-culture time for callus inducement of stem section explants is 11 to 15 days ; that the OD600 value of bacterium inoculum solution is between 0. 5 and 0.8; and that the co-culture time is 13 days and pH value of co-culture medium is 5, 6 to 6.2. Re suspension of inoculum solution enhances the transformation efficiency but the addition of Actosyringone does not improve it, Different clones have different susceptibility to the infection of LBA4404 and clones with high susceptibility should be selected to do the transformation breeding of teak.