文献类型：期刊文献

英文题名：Ubiquitinated DA1 negatively regulates vascular cambium activity through modulating the stability of WOX4 in Populus

作者：Tang, Xianfeng[1] Wang, Congpeng[2] Chai, Guohua[3] Wang, Dian[2] Xu, Hua[1] Liu, Yu[2] He, Guo[1] Liu, Shuqing[1] Zhang, Yiran[3] Kong, Yingzhen[2] Li, Shengjun[1] Lu, Mengzhu[4] Sederoff, Ronald[5] Li, Quanzi[6] Zhou, Gongke[2]

第一作者：Tang, Xianfeng

通信作者：Zhou, GK[1];Li, QZ[2]

机构：[1]Chinese Acad Sci, Shandong Inst Energy Technol, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels,Shandong Prov Key Lab Energy Gen, Qingdao 266101, Peoples R China;[2]Qingdao Agr Univ, Coll Landscape Architecture & Forestry, Qingdao 266109, Shandong, Peoples R China;[3]Qingdao Agr Univ, Coll Resources & Environm, Qingdao 266109, Peoples R China;[4]Zhejiang Agr & Forestry Univ, Coll Forestry & Biotechnol, Hangzhou 311300, Peoples R China;[5]North Carolina State Univ, Dept Forestry & Environm Resources, Forest Biotechnol Grp, Raleigh, NC 27695 USA;[6]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：0

外文期刊名：PLANT CELL

收录：;Scopus(收录号：2-s2.0-85137134775);WOS:【SCI-EXPANDED(收录号:WOS:000817126800001)】；

基金：This work was supported by the National Key Scientific Research Project of China (2021YFD2200205), the National Natural Science Foundation of China (32071725, 31972860 and 31700526), the First Class Grassland Science Discipline Program of Shandong Province, and Shandong Energy Institute (SEI I202130 to S.L.).

语种：英文

摘要：A ubiquitin receptor highly expressed in the vascular cambium destabilizes the WUSCHEL-related HOMEOBOX protein WOX4 in a ubiquitin-dependent manner to regulate cambial cell division in Populus. Activity of the vascular cambium gives rise to secondary xylem for wood formation in trees. The transcription factor WUSCHEL-related HOMEOBOX4 (WOX4) is a central regulator downstream of the hormone and peptide signaling pathways that maintain cambial activity. However, the genetic regulatory network underlying WOX4-mediated wood formation at the post-transcriptional level remains to be elucidated. In this study, we identified the ubiquitin receptor PagDA1 in hybrid poplar (Populus alba x Populus glandulosa clone 84K) as a negative regulator of wood formation, which restricts cambial activity during secondary growth. Overexpression of PagDA1 in poplar resulted in a relatively reduced xylem due to decreased cambial cell division. By contrast, mutation of PagDA1 by CRISPR/Cas9 resulted in an increased cambial cell activity and promoted xylem formation. Genetic analysis demonstrated that PagDA1 functions antagonistically in a common pathway as PagWOX4 to regulate cambial activity. We propose that PagDA1 physically associates with PagWOX4 and modulates the degradation of PagWOX4 by the 26S proteasome. Moreover, genetic analysis revealed that PagDA1 exerts its negative effect on cambial development by modulating the stability of PagWOX4 in a ubiquitin-dependent manner mediated by the E3 ubiquitin ligase PagDA2. In sum, we have identified a cambial regulatory protein complex, PagDA1-PagWOX4, as a potential target for wood biomass improvement.