文献类型：期刊文献

中文题名：泡泡刺高通量转录组鉴定及其黄酮类代谢途径初步分析

英文题名：High-throughput Transcriptome Identification and Flavonoids Metabolic Pathways in Nitraria sphaerocarpa

作者：马婧[1] 邓楠[1] 褚建民[1] 纪敬[1] 史胜青[1] 江泽平[1] 成铁龙[1,2]

第一作者：马婧

机构：[1]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091;[2]南京林业大学,江苏南京210037

年份：2016

卷号：29

期号：1

起止页码：61-66

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家自然科学基金(31270707;31100490)

语种：中文

中文关键词：泡泡刺;转录组;RNA-seq;黄酮类化合物

外文关键词：Nitraria sphaerocarpa; transcriptome; RNA-seq; flavonoids

分类号：S718.5

摘要：[目的]为了更好的了解泡泡刺黄酮类生物合成途径及其潜在的抗逆作用,[方法]应用高通量RNA-seq测序技术对泡泡刺当年生新叶进行了转录组测序及相关生物信息学分析。[结果]表明:泡泡刺当年生叶中获得了13 013 444 total reads,总核苷酸数为1 171 209 960 bp(1.09 Gb),组装拼接后得到48 921条unigene序列;Nr、SwissProt、COG、GO、KEGG等数据库分析显示,有30 407个Nr注释,19 671个Swissprot注释,9 273个COG功能注释,46 153个GO功能注释,13 654个KEGG注释,并从KEGG通路中找到参与黄酮类化合物合成途径的关键基因片段186个。[结论]本研究获得了较好的泡泡刺转录组序列信息,并且其中富含黄酮类化合物代谢途径的相关基因,这为其抗逆机制、药用价值的研究及开发利用提供宝贵资源。
[Objective]To understand the biosynthetic pathway of flavonoids and its roles in the adaptation to abiotic stresses in Nitraria sphaerocarpa. [Method]High-throughput RNA-seq technology was used to generate the transcriptome of current-year leaves,and then the related bioinformatics analysis was performed. [Result]The results showed that 13 013 444 raw reads and 48 921 unigenes were acquired after de novo assembly; 30 407 annotations were acquired from Nr database,19 671 from Swiss-Prot database,9 273 from COG database,46 153 from GO database and 13 654 from KEGG database after function annotation against five databases; 186 unigenes were associated with flavonoid biosynthesisby the analysis of KEGG pathway. [Conclusion]The better transcriptomic data was obtained,and it was rich in the genes involved in flavonoids biosynthesis,which would provide references for the studies of its resistance,medicinal value in future.