文献类型：期刊文献

中文题名：基于表型和SSR标记的陇南油橄榄品种鉴定与遗传多样性分析

英文题名：Identification and Genetic Diversity Analysis of Olive Cultivars in Longnan Based on Phenotype and SSR Markers

作者：徐悦[1] 黄兰[1] 李金花[1] 邓煜[2] 张建国[1] 曾艳飞[1]

第一作者：徐悦

机构：[1]国家林业和草原局林木培育重点实验室,中国林业科学研究院林业研究所,北京100091;[2]甘肃省陇南市经济林研究院油橄榄研究所,甘肃陇南746000

年份：2022

卷号：35

期号：4

起止页码：33-43

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家重点研发计划项目课题(2019YFD1000602)。

语种：中文

中文关键词：油橄榄;表型多样性;质量性状;数量性状;品种鉴定;遗传多样性

外文关键词：olive;phenotypic diversity;quality trait;quantitative trait;variety identification;genetic diversity

分类号：S722.3

摘要：[目的]该研究对我国油橄榄主产区甘肃省陇南市现有品种进行了的准确鉴定和遗传多样性计算,为我国油橄榄种质资源引种和驯化、育种及利用提供参考。[方法]利用13个SSR标记对来自83个油橄榄品种的110棵单株进行品种鉴定和遗传多样性分析,并对其中属于68个品种的90棵单株进行叶片、果实和果核形态性状的测定和分析,最后开展了基于表型性状和SSR标记的单株聚类和结果比较。[结果]陇南油橄榄表型数量性状和质量性状多样性指数分别为1.83和0.79;SSR分析发现83个品种包括78个不同的基因型,观测杂合度和期望杂合度分别为0.683和0.754;这些基因型可分为3大组,基因型所属分组与其起源地有一定相关性;我国选育品种的基因型大多与起源意大利的品种分组更近;与基于表型的聚类树相比,基于SSR基因型的聚类树更倾向于将同一品种的不同单株聚在同一支。[结论]陇南共有78种不同基因型的品种,SSR标记比表型鉴定品种更具准确性,且能反映亲缘关系。该地区油橄榄表型多样性和遗传多样性较高,引种品种主要是来自地中海西部和中部国家,国内选育品种与意大利起源的品种相似;为更多样化可考虑引种或选育更多起源国的品种。
[Objective]This study accurately identified the existing varieties and calculated genetic diversity in Longnan City,Gansu Province,which was the main olive producing area in China,and provided a reference for the introduction,domestication,breeding and utilization of olive germplasm resources in China.[Method]Identification and genetic diversity were analyzed for 110 individuals of 83 olive varieties collected from Longnan city using 13 SSR markers,and phenotypic diversity was analyzed with indicators such as leaf,fruit,and stone for 90 individuals of 68 varieties.And the clustering of varieties based on phenotypes was compared with that based on SSR markers.[Result]The phenotypic diversity indices for olive varieties from Longnan were 1.83 for quantitative traits and 0.79 for quality traits,respectively;SSR markers identified 78 different genotypes for all olives analyzed,with an observed heterozygosity of 0.683 and an expected heterozygosity of 0.754,respectively;all varieties could be divided into three groups,and the grouping of varieties were more or less related with their origin;most of the varieties bred and selected in China were closer to those originated from Italy;compared with those in the phenotypic-based cluster tree,different individuals of the same variety were more inclined to cluster together in the tree based on SSR genotypes.[Conclusion]In Longnan,78 varieties with different genotypes were identified based on SSR markers,which shows a higher accuracy in variety identification and a clear kinship among varieties than that based on phenotypes.The phenotypic diversity and genetic diversity of olive in Longnan are high,and the introduced varieties are mainly from the western and central Mediterranean countries.Whereas the varieties bred and selected previously in China are more similar to varieties originated from Italy.To diversifying olive germplasm in Longnan,more varieties should be introduced and bred with varieties from different countries.