文献类型：期刊文献

英文题名：Chromosome-level genome of Camellia lanceoleosa provides a valuable resource for understanding genome evolution and self-incompatibility

作者：Gong, Wenfang[1,2] Xiao, Shixin[1,2] Wang, Linkai[1,2] Liao, Zhenyang[3] Chang, Yihong[1,2] Mo, Wenjuan[4,5] Hu, Guanxing[1,2] Li, Wenying[1,2] Zhao, Guang[1,2] Zhu, Huaguo[6] Hu, Xiaoming[6] Ji, Ke[1,2] Xiang, Xiaofeng[1,2] Song, Qiling[1,2] Yuan, Deyi[1,2] Jin, Shuangxia[7] Zhang, Lin[1,2]

第一作者：Gong, Wenfang

通信作者：Yuan, DY[1];Zhang, L[1];Yuan, DY[2];Zhang, L[2];Jin, SX[3]

机构：[1]Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China;[2]Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod, Forestry Minist, Changsha 410004, Hunan, Peoples R China;[3]Chinese Acad Agr Sci, Genome Anal Lab, Minist Agr,Agr Genom Inst Shenzhen, Shenzhen Branch,Guangdong Lab Lingnan Modern Agr, Shenzhen 518120, Peoples R China;[4]Chinese Acad Forestry, Expt Ctr Forestry North China, Natl Permanent Sci Res Base Warm Temperate Zone F, Beijing 102300, Peoples R China;[5]Cornell Univ, Coll Agr & Life Sci, Sch Integrat Plant Sci, Ithaca, NY 14853 USA;[6]Huanggang Normal Univ, Coll Biol & Agr Resources, Huanggang 438000, Hubei, Peoples R China;[7]Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China

年份：2022

卷号：110

期号：3

起止页码：881-898

外文期刊名：PLANT JOURNAL

收录：;EI(收录号：20221712032357);Scopus(收录号：2-s2.0-85128407040);WOS:【SCI-EXPANDED(收录号:WOS:000787317900001)】；

基金：Our work was supported by the National Key R&D Program of China (2018YFD1000603-1), the Natural Science Foundation of Hunan Province (2020JJ5968), Scientific Research Foundation for Advanced Talents of Central South University of Forestry and Technology (2018YJ002), the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (CAFYBB2018GB001), and the Key Program of Education Department of Hunan Province (grant no. 20A524).

语种：英文

外文关键词：Camellia lanceoleosa; chromosome-level genome; oil synthesis; caffeine distribution; self-incompatibility

摘要：The section Oleifera (Theaceae) has attracted attention for the high levels of unsaturated fatty acids found in its seeds. Here, we report the chromosome-scale genome of the sect. Oleifera using diploid wild Camellia lanceoleosa with a final size of 3.00 Gb and an N50 scaffold size of 186.43 Mb. Repetitive sequences accounted for 80.63% and were distributed unevenly across the genome. Camellia lanceoleosa underwent a whole-genome duplication event approximately 65 million years ago (65 Mya), prior to the divergence of C. lanceoleosa and Camellia sinensis (approx. 6-7 Mya). Syntenic comparisons of these two species elucidated the genomic rearrangement, appearing to be driven in part by the activity of transposable elements. The expanded and positively selected genes in C. lanceoleosa were significantly enriched in oil biosynthesis, and the expansion of homomeric acetyl-coenzyme A carboxylase (ACCase) genes and the seed-biased expression of genes encoding heteromeric ACCase, diacylglycerol acyltransferase, glyceraldehyde-3-phosphate dehydrogenase and stearoyl-ACP desaturase could be of primary importance for the high oil and oleic acid content found in C. lanceoleosa. Theanine and catechins were present in the leaves of C. lanceoleosa. However, caffeine can not be dectected in the leaves but was abundant in the seeds and roots. The functional and transcriptional divergence of genes encoding SAM-dependent N-methyltransferases may be associated with caffeine accumulation and distribution. Gene expression profiles, structural composition and chromosomal location suggest that the late-acting self-incompatibility of C. lanceoleosa is likely to have favoured a novel mechanism co-occurring with gametophytic self-incompatibility. This study provides valuable resources for quantitative and qualitative improvements and genome assembly of polyploid plants in sect. Oleifera.