文献类型：期刊文献

英文题名：Alcohol dehydrogenases regulated by a MYB44 transcription factor underlie Lauraceae citral biosynthesis

作者：Zhao, Yunxiao[1,2] Chen, Yicun[1,2] Gao, Ming[1,2] Wang, Yangdong[1,2]

第一作者：赵耘霄

通信作者：Wang, YD[1];Wang, YD[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China

年份：2023

外文期刊名：PLANT PHYSIOLOGY

收录：;Scopus(收录号：2-s2.0-85186744240);WOS:【SCI-EXPANDED(收录号:WOS:001100509400001)】；

基金：We are highly thankful to Professor Zhilin Yuan, Hengfu Yin and Zhenhua Liu, Dr. Minyan Wang, and Bin Liu for their helpful suggestions.

语种：英文

摘要：Lineage-specific terpenoids have arisen throughout the evolution of land plants and are believed to play a role in interactions between plants and the environment. Species-specific gene clusters in plants have provided insight on the evolution of secondary metabolism. Lauraceae is an ecologically important plant family whose members are also of considerable economic value given their monoterpene contents. However, the gene cluster responsible for the biosynthesis of monoterpenes remains yet to be elucidated. Here, a Lauraceae-specific citral biosynthetic gene cluster (CGC) was identified and investigated using a multifaceted approach that combined phylogenetic, collinearity, and biochemical analyses. The CGC comprises MYB44 as a regulator and 2 alcohol dehydrogenases (ADHs) as modifying enzymes, which derived from species-specific tandem and proximal duplication events. Activity and substrate divergence of the ADHs has resulted in the fruit of mountain pepper (Litsea cubeba), a core Lauraceae species, consisting of more than 80% citral. In addition, MYB44 negatively regulates citral biosynthesis by directly binding to the promoters of the ADH-encoding genes. The aggregation of citral biosynthetic pathways suggests that they may form the basis of important characteristics that enhance adaptability. The findings of this study provide insights into the evolution of and the regulatory mechanisms involved in plant terpene biosynthesis. Lauraceae plants are abundant in citral due to the formation of lineage-specific clusters of terpenoid synthesis genes, which include transcription factor and modifying enzymes.