文献类型：期刊文献

英文题名：LcMYB43 enhances monoterpene biosynthesis by activating 1-deoxy-D-xylulose-5-phosphate synthase gene expression in Litsea cubeba

作者：Ni, Feifei[1,2,3] Zhao, Yunxiao[1,3] Gao, Ming[1,3] Chen, Yicun[1,3] Wang, Yangdong[1,3]

第一作者：Ni, Feifei

通信作者：Chen, YC[1];Wang, YD[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Nanjing 210037, Peoples R China;[3]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China

年份：2025

卷号：304

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20250717858315);Scopus(收录号：2-s2.0-85217275234);WOS:【SCI-EXPANDED(收录号:WOS:001426757300001)】；

基金：This work was supported by National Natural Science Foundation of China (32471922) ; Zhejiang Province Key Research and Development Program (2024C02009) .

语种：英文

外文关键词：MYB; DXS; Monoterpene biosynthesis

摘要：MYB transcription factors are crucial regulators involved in various metabolic processes in plants, including terpene biosynthesis. Litsea cubeba, a member of the Lauraceae family, is rich in monoterpenes and regulates their biosynthesis via the key enzyme DXS in the MEP pathway. Seven DXS genes have been identified in this species, but the role of the MYB family in terpene biosynthesis remains unclear. This study conducted a genomewide characterization of the R2R3-MYB gene family in L. cubeba, analyzing its phylogenetics, expression, and regulatory functions. A total of 129 R2R3-MYB members were identified, with expansion mechanisms involving tandem and segmental duplications. Expression analysis revealed that LcMYB43 activates LcDXS5, a key enzyme in monoterpene biosynthesis. Overexpression of LcMYB43 significantly increased monoterpene accumulation. Y1H, EMSA, and dual-luciferase assays showed that LcMYB43 directly binds to the CAACAG motif in the LcDXS5 promoter, activating its expression. These findings suggest that LcMYB43 enhances monoterpene biosynthesis by promoting LcDXS5 expression, providing new insights into the regulatory mechanisms of monoterpene biosynthesis.