文献类型：期刊文献

英文题名：Genetic Analysis of Walnut Cultivars in China Using Fluorescent Amplified Fragment Length Polymorphism

作者：Ma, Qingguo[1] Zhang, Junpei[1] Pei, Dong[1]

第一作者：马庆国

通信作者：Pei, D[1]

机构：[1]Chinese Acad Forestry, Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2011

卷号：136

期号：6

起止页码：422-428

外文期刊名：JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE

收录：;Scopus(收录号：2-s2.0-81755162258);WOS:【SCI-EXPANDED(收录号:WOS:000297599100007)】；

基金：We gratefully acknowledge financial support from the '948' Project (No.2009-4-07) and the key project of the state forestry administration, P.R. China (No.2008-04). The contributions of Meiyong Zhang, Shangfeng Gao, Jing Qi, and Ying Gao are also acknowledged with respect to their assistance on sample collections.

语种：英文

外文关键词：genetic diversity; relationship; Juglans regia

摘要：Informative DNA fingerprints from 50 walnut cultivars (Juglans regia) in China were generated using amplified fragment length polymorphism (AFLP) markers to reveal their genetic diversity and relationships. Nine primer combinations were selected from 64 EcoRI/Mse I primer combinations to amplify the accessions. An average of 132 polymorphic loci per primer set was detected from the nine primer combinations. The discrimination power of each polymorphic marker (estimated by the polymorphism information content) ranged from 0.00 to 0.37 with an average of 0.19. A moderate level of genetic diversity was observed among the 50 cultivars. Their expected heterozygosity varied from 0.38 to 0.50 (average, 0.44), and Dice's similarity coefficient ranged from 0.53 to 0.86 (average, 0.70). The cluster analysis conducted using the unweighted pair group method of arithmetic averages method showed that all of the cultivars fell into five groups at Dice's similarity coefficient of 0.68. According to the comprehensive analyses based on the specific loci, similarity coefficient, and clustering results, six cultivars (Liaoning 1, Zixin, Shanhe 4, Zha 343, Tulare, and Chandler) were considered important germplasms of walnut cultivars.