文献类型：期刊文献

英文题名：Functional significance of asymmetrical retention of parental alleles in a hybrid pine species complex

作者：Qu, Chang[1] Kao, Hong-Na[1] Xu, Hui[1] Wang, Bao-Sheng[2] Yang, Zhi-Ling[3] Yang, Qi[4] Liu, Gui-Feng[1] Wang, Xiao-Ru[5] Liu, Yan-Jing[1] Zeng, Qing-Yin[1]

第一作者：Qu, Chang

通信作者：Liu, YJ[1];Zeng, QY[1]

机构：[1]Northeast Forestry Univ, Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable Ut, South China Bot Garden, Guangzhou 510650, Peoples R China;[3]Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Menglun 666303, Yunnan, Peoples R China;[4]Zhejiang A&F Univ, Coll Forestry & Biotechnol, State Key Lab Subtrop Silviculture, Hangzhou 311300, Zhejiang, Peoples R China;[5]Umea Univ, Dept Ecol & Environm Sci, UPSC, SE-90187 Umea, Sweden

年份：0

外文期刊名：JOURNAL OF SYSTEMATICS AND EVOLUTION

收录：;Scopus(收录号：2-s2.0-85152362299);WOS:【SCI-EXPANDED(收录号:WOS:000970443700001)】；

基金：We thank the staff at beamline BL19U of the SSRF for their assistance with data collection, and the staff at the X-ray crystallography platform of the Tsinghua University Technology Center for Protein Research for providing us with facility support. This study was supported by the Fundamental Research Funds for the Central Non-profit Research Institution of the Chinese Academy of Forestry (CAFYBB2019ZY002, TGB2020001, TGB2021001, TGB2022001).

语种：英文

外文关键词：enzymatic function; functional divergence; gene expression; glutathione S-transferases; homoploid hybrid species

摘要：Hybrid genomes usually harbor asymmetrical parental contributions. However, it is challenging to infer the functional significance of asymmetrical retention of parental alleles in hybrid populations of conifer trees. Here we investigated the diversity in the glutathione S-transferase (GST) gene family in a hybrid pine Pinus densata and its parents (Pinus tabuliformis and Pinus yunnanensis). Plant GSTs play major roles in protecting plants against biotic and abiotic stresses. In this study, 19 orthologous groups of GST genes were identified and cloned from these three species. We examined their expression in difFerent tissues, and then purified the corresponding proteins to characterize their enzymatic activities and specificities toward difFerent substrates. We found that among the 19 GST orthologous groups, divergence in gene expression and in enzymatic activities toward difFerent substrates was prevalent. P. densata preferentially retained P. yunnanensis-like GSTs for 17 out of the 19 gene loci. We determined the first GST crystal structure from conifer species at a resolution of 2.19 A. Based on this structure, we performed site-directed mutagenesis to replace amino acid residuals in difFerent wild-types of GSTs to understand their functional impacts. Reciprocal replacement of amino acid residuals in native GSTs of P. densata and P. tabuliformis demonstrated significant changes in enzyme functions and identified key sites controlling GSTs activities. This study illustrates an approach to evaluating the functional significance of sequence variations in conifer genomes. Our study also sheds light on plausible mechanisms for controlling the selective retention of parental alleles in the P. densata genome.