文献类型：期刊文献

中文题名：增强贝莱斯芽胞杆菌CLA178根际定殖的蔷薇根系分泌组分鉴定

英文题名：Root exudates in Rosa-microbe interaction contribute to the increased colonization of Bacillus velezensis CLA178

作者：陈淋[1] 王兴红[1] 刘云鹏[2]

第一作者：陈淋

机构：[1]中国林业科学研究院华北林业实验中心,北京102300;[2]中国农业科学院农业资源与农业区化研究所,北京100081

年份：2021

卷号：44

期号：3

起止页码：497-505

中文期刊名：南京农业大学学报

外文期刊名：Journal of Nanjing Agricultural University

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：中国林业科学研究院基本科研业务费专项资助项目(CAFYBB2017MA020);国家自然科学基金项目(31700548)。

语种：中文

中文关键词：蔷薇;根系分泌物;贝莱斯芽胞杆菌;趋化;生物膜;根际定殖

外文关键词：rose;root exudates;Bacillus velezensis;chemotaxis;biofilm;root colonization

分类号：S182

摘要：[目的]根系分泌物在植物-微生物根际互作过程中起到关键作用,本文旨在深入了解根际促生菌与蔷薇植物之间的根际互作机制。[方法]通过分根系统检测预接种益生菌或病原菌对植物根系分泌物组分和根际促生菌贝莱斯芽胞杆菌(Bacillus velezensis)CLA178菌株定殖的影响;通过分析不同处理的蔷薇根际分泌物组分与菌株定殖的相关性,筛选潜在的信号物质;通过检测不同物质与CLA178菌株趋化性、生物膜形成、生长、定殖及相关基因表达的关系,确定调控菌株CLA178定殖的根际信号物质。[结果]预接种CLA178或根癌农杆菌(Agrobacterium tumefaciens)C58均会促进CLA178定殖。根系分泌物中的葡萄糖酸(GLcA)和海藻糖-6-磷酸(T6P)的含量与CLA178的趋化性、生物膜形成、根际定殖和epsD、yqxM、kinC基因表达均呈正相关关系,20~100μmol·L^(-1) GLcA和T6P分别显著提高CLA178的根际定殖;而CLA178对邻苯二甲酸二辛酯(DOP)无显著趋化响应,但是该物质与CLA178的生长、生物膜形成、定殖呈负相关。[结论]预接种贝莱斯芽胞杆菌CLA178和根癌农杆菌C58后,蔷薇根系分泌物中GLcA和T6P含量增加,DOP含量降低,参与互作过程中益生菌CLA178根际定殖增强,进而协助植物防控病害。
[Objectives]Root exudates play an important role in plant-microbe rhizosphere interactions.This paper was aimed to further understand the rose root exudates-mediated interaction between rose and plant growth-promoting rhizobacteria(PGPR).[Methods]The impacts of preinoculation with beneficial bacteria or pathogens on root exudates and subsequent colonization by PGPR Bacillus velezensis CLA178 were investigated in a split-root system.The potential signal compounds were selected through analyzing the correlation between root exudates and root colonization by CLA178.The signal compounds in root exudates were determined by measuring the effects of different compounds on the chemotaxis,biofilm formation,growth,colonization and related genes expression of CLA178.[Results]Preinoculation of rose plants in a split-root system with B.velezensis CLA178 or pathogen Agrobacterium tumefaciens C58 inhibited the subsequent colonization by CLA178.The root secretion of gluconic acid(GLcA)and trehalose-6-phosphate(T6P)had positive effects on the chemotaxis,biofilm formation,colonization and expression of epsD,yqxM,and kinC genes of CLA178.The colonization of CLA178 was significantly enhanced by 20-100μmol·L^(-1) GLcA and T6P,respectively.The root secretion of dioctyl phthalate(DOP)had no significant effect on the chemotaxis of CLA178,but had negative effects on the growth,biofilm formation and colonization of CLA178.[Conclusions]Rose plants enhanced the root secretion of GLcA and T6P,and reduced the root secretion of DOP in response to microbial colonization,thereby increasing the colonization of B.velezensis CLA178 to assist plant in disease resistance.