文献类型：期刊文献

英文题名：Genetic diversity of natural populations of Taxus mairei

作者：Luo, Qianqian[1,2,3] Li, Fengqing[4] Yu, Longhua[4] Wang, Liyun[4] Xu, Gangbiao[5] Zhou, Zhichun[1,2]

第一作者：Luo, Qianqian

通信作者：Zhou, ZC[1];Zhou, ZC[2]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Daqiao Rd 73, Hangzhou 311400, Peoples R China;[2]Zhejiang Prov Key Lab Tree Breeding, Daqiao Rd 73, Hangzhou 311400, Peoples R China;[3]Forestry Res Inst Fuzhou, Fuzhou 344000, Peoples R China;[4]Chinese Acad Forestry, Expt Ctr Subtrop Forestry, Fenyi 336600, Peoples R China;[5]Cent South Univ Forestry & Technol, Coll Forestry, Changsha 410004, Peoples R China

年份：2022

卷号：23

期号：1

起止页码：63-74

外文期刊名：CONSERVATION GENETICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000702800900001)】；

基金：We thank Professor Zhichun Zhou for helpful suggestions and arrangements for the experiments performed at Zhejiang Provincial Key Laboratory of Tree Breeding, Hangzhou, P. R. China. This research was funded by fundamental Research Funds of the Central Public Welfare Research Institutes, Chinese Academy of Forestry (No.CAFYBB2018ZB010). Samples were collected by Longhua Yu, Liyun Wang, and Fengqing Li.

语种：英文

外文关键词：Taxus mairei; Genetic diversity; Natural populations; SSR

摘要：Taxus mairei (Lemee & Lev.) S.Y. Hu ex T. S. Liu is a vulnerable tree species, and it is also a precious timber species in China. We used 13 microsatellites to assess the genetic diversity and differentiation of 665 T. mairei samples from 18 natural populations. A total of 291 alleles were detected. The average number of alleles (N-a = 22.39), expected heterozygosity (H-e = 0.74), polymorphic information content (PIC = 0.86) and Shannon diversity index (I = 1.66) of the loci indicated a high level of genetic diversity in natural T. mairei populations. Moreover, gene flow was more active among populations (N-m = 1.62) than within populations. Among the 18 populations, the Xinfeng population in Jiangxi Province has the highest genetic diversity. Although each of the studied populations should be protected from further deforestation and agricultural expansion, the Xinfeng population deserves the highest conservation priority. The results based on analysis of molecular variance showed that genetic variation occurred mainly within populations (84.90%; P < 0.001), which indicated that the degree of genetic differentiation of the natural populations of T. mairei was low. Based on UPGMA, the 18 populations were categorized into two groups. A Mantel test showed that there was no significant correlation between standard genetic distance and geographical distance or altitude differences among the populations. The genetic clustering results also indicated that there are varying degrees of gene penetration among natural populations of T. mairei. The information presented here forms the basis for the development of genetic guidelines for appropriate conservation programs.