文献类型：期刊文献

中文题名：马大杂种相思瓶内和瓶外生根技术研究

英文题名：In Vitro and ex Vitro Rooting Technology Comparison of Acacia mangium × A. auriculiformis hybrids

作者：施琼[1] 胡峰[2] 黄烈健[1]

第一作者：施琼

机构：[1]中国林业科学研究院热带林业研究所;[2]广东省农业科学院作物研究所

年份：2015

卷号：35

期号：6

起止页码：891-897

中文期刊名：植物研究

外文期刊名：Bulletin of Botanical Research

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家"十二五"科技支撑计划项目(No.2012BAD01B0402)

语种：中文

中文关键词：马大杂种相思;瓶内生根;瓶外生根;经济效益

外文关键词：Acacia mangium × A. auriculiformis;in vitro rooting;ex vitro rooting;economic benefit

分类号：Q813.1

摘要：以增殖培养的马大杂种相思无菌苗为材料，进行瓶内生根（采用增殖培养30d，2～3cm的无菌苗）和瓶外生根（采用增殖培养45d，3～5cm的无菌苗）技术的研究，并对2种生根技术进行了经济效益的简单比较分析。结果表明：将增殖培养30d的马大杂种相思无菌苗接入1／2MS＋IBA 1．0mg·L^-1＋NAA 0．5mg·L^-1＋蔗糖30％的培养基中，生根率可达99．43％，15d后移栽，存活率达98．0％；而将增殖培养45d的马大杂种相思无菌苗用100mg·L^-1的IBA溶液浸泡1h进行瓶外生根实验，生根率可达94．67％。按生产10000株苗计算，瓶外生根的经济效益比瓶内生根的多1288．26元，效益比较可观，瓶外生根技术可以应用于马大杂种相思组培苗的大量生产。
For improving the rooting percentage and providing an optimal technology for seedling production, we studied the rooting technology of in vitro and ex vitro of Acacia mangium x A. auriculiformis hybrid by using the proliferation seedlings as materials （in vitro rooting with the proliferation cultured for 30 d, 2 - 3 cm seedling; and ex vitro rooting with the proliferation cultured for 45 d, 3 - 5 cm seedlings）, and compared the economic benefits of two techniques for seedling production. Inoculating the aseptic plants which had been multiplication cultured for 30 d to 1/2MS with combination of 1.0 mg ·L^-1 IBA, 0.5 mg·L^-1 NAA, 3% sucrose could get the highest rooting rate of 99.43%, and the survival rate was 98.0% after transplanted to the greenhouse 15 d. However, when immersing the aseptic seedlings which had been muhiphcation cultured for 45 d with 100 mg · L^-1 IBA for 1 hour, the rooting rate was 94.67%. For producing 10 000 seedlings, the mangium × A. auriculiformis.