文献类型：期刊文献

中文题名：延缓生长法在试管内保存牛皮杜鹃种质初探

英文题名：Preliminary Study on Germplasm Conservation in vitro of Rhododendron chrysanthum Pall.through Deferring Growth

作者：顾地周[1] 杨丽娟[1] 冯颖[1] 姜云天[1] 高捍东[2] 邱德有[3]

第一作者：顾地周

机构：[1]通化师范学院生物系;[2]南京林业大学森林资源与环境学院;[3]中国林业科学研究院林业研究所

年份：2011

卷号：27

期号：4

起止页码：546-548

中文期刊名：科技通报

外文期刊名：Bulletin of Science and Technology

收录：CSTPCD;;北大核心:【北大核心2008】；

基金：吉林省教育厅科学技术研究项目资助(吉教科合字[2009]第270号)

语种：中文

中文关键词：牛皮杜鹃;延缓生长;试管保存;根皮苷;均匀设计

外文关键词：Rhododendron chrysanthum Pall. ; deferring growth; conservation in vitro; phlorizin; uniform design

分类号：Q813.1

摘要：以牛皮杜鹃嫩茎基部直接再生形成的丛生芽团为试材,应用均匀设计法探讨了影响牛皮杜鹃丛生芽团试管保存的主要因子和水平,同时筛选最适于牛皮杜鹃丛生芽团试管保存的培养基,结果表明:最适宜的丛生芽团试管保存培养基为:DR+根皮苷2.70 mg/L+KT0.70 mg/L,保存46个月生长率仅为1.12%。对保存前后的芽苗分化的结果进行对比可知,分化率达99.5%以上,较未保存前的分化率89.0%高,且保存后的生根速度快。本试验结果证明,在试管内采取"延缓生长"法保存牛皮杜鹃丛生芽团是切实可行的新方法。
In this study,the adventitious buds induced from the tender stems of Rhododendron chrysanthum Pall. was used as explant and the main factors with different levels for germplasm conservation in vitro were investigated through uniform design experiments,and to screen the appropriate medium for in vitro conservation the adventitious buds of Rhododendron chrysanthum Pall. The results showed that DR＋phloridzin2.70 mg/L＋KT0.70 mg/L was suitable for in vitro conservation of the adventitious buds, the rate of regeneration was 1.12% in 46 months. The contrast test results of redifferentiation of before and after in vitro conservation of the adventitious buds, the rate of differentiation was more than 99.5% ,which is higher differentiation rate than 89.0% before in vitro corrservation, and shoots take root quickly after in vitro conservation. The experiment results proved that in vitro conservation the adventitious buds of Rhododerutron chrysanthum Pall. is feasible new method by defering growth.